During the staining of sputum smear of a patient with suspected croupous pneumonia the following reactives and stainers were used: gentian violet solution, Lugol's solution, 960 spiritus and water fuchsin. What method of staining is used in this case?

Correct Answer: B

Rationale: The correct answer is B: Gram's staining method. The rationale for this is as follows: Gentian violet and Lugol's solution are used as primary and mordant stains in the Gram's staining method. Spiritus 960 is used as a decolorizer, and water fuchsin is used as a counterstain. This staining method is commonly used in microbiology to differentiate bacteria into Gram-positive and Gram-negative based on their cell wall characteristics. A: Leffler's staining method is used for the identification of diphtheria bacilli. C: Ziehl-Neelsen's staining method is used for acid-fast bacteria like Mycobacterium tuberculosis. D: Neisser's staining method is used for the identification of Neisseria species. In summary, the use of specific reactives and stainers in the question indicates the application of the Gram's staining method for bacterial differentiation.

Correct Answer: C

Rationale: The correct answer is C: Candida albicans. Candida albicans is a common fungal organism that causes diaper rash in infants due to its ability to thrive in warm, moist environments. It can overgrow in the diaper area, leading to irritation and inflammation. Sporothrix schenckii (A) is a fungus that causes skin infections, but not typically diaper rash. Actinomyces (B) is a bacterium that is not commonly associated with diaper rash. Malassezia furfur (D) is a yeast that causes conditions like dandruff and seborrheic dermatitis, but not diaper rash in infants.

Correct Answer: A

Rationale: The correct answer is A: Parvoviridae. Parvoviruses are DNA viruses, as their genetic material is composed of DNA. This distinguishes them from RNA viruses like Picornaviridae, Astroviridae, and Togaviridae. Parvoviruses replicate in the host cell nucleus, further confirming their DNA nature. Therefore, based on their DNA composition and replication process, Parvoviridae is the correct choice.

Correct Answer: A

Rationale: The correct answer is A: Staphylococcus aureus. Staphylococcus aureus is a Gram-positive bacterium that commonly presents as cocci in clusters. It is a known causative agent of abscesses due to its ability to produce enzymes and toxins that contribute to tissue destruction. In this case, the presence of Gram-positive cocci in clusters aligns with the characteristic morphology of Staphylococcus aureus. Summary of incorrect choices: B: Streptococcus pyogenes is a Gram-positive bacterium that typically presents as cocci in chains, not clusters. It is more commonly associated with pharyngitis and skin infections rather than abscesses. C: Enterococcus faecalis is a Gram-positive bacterium that typically presents as cocci in pairs or short chains, not clusters. It is more commonly associated with urinary tract infections and endocarditis. D: Micrococcus luteus is a Gram-positive bacterium that typically presents as t

Correct Answer: D

Rationale: The correct answer is D: reemerging diseases. Group II category diseases by the NIAID are those that have reemerged or have the potential to reemerge. This classification helps prioritize research and resources. Choice A is incorrect because new foodborne pathogens fall under different categories. Choice B is incorrect as eradicated diseases are not part of the reemerging group. Choice C is incorrect because bioterrorism agents are classified separately. Therefore, the correct answer is D as it aligns with the purpose of Group II category diseases.

Correct Answer: C

Rationale: The correct answer is C: Vibrio. Cholera is caused by Vibrio cholerae bacteria. These bacteria produce a toxin that causes severe diarrhea and dehydration. Escherichia (choice A) and Salmonella (choice B) are known for causing other types of gastrointestinal infections but not cholera. Acinetobacter (choice D) typically causes respiratory infections, not gastrointestinal illnesses like cholera. Therefore, the correct choice is Vibrio as it is specifically associated with cholera.

Correct Answer: C

Rationale: The correct answer is C: adhesion to surfaces and conjugation. Pili are hair-like structures on the surface of bacteria that help them adhere to surfaces and facilitate the transfer of genetic material during conjugation. This is essential for bacterial survival and reproduction. Choice A is incorrect because protection from desiccation is typically associated with other structures like capsules. Choice B is incorrect as motility in bacteria is usually facilitated by flagella. Choice D is incorrect as spore formation involves the formation of specialized structures, not pili.

Correct Answer: A

Rationale: Step 1: Treponema pallidum is a spirochete bacterium known to cause syphilis. Step 2: It is sexually transmitted and can cause various stages of disease. Step 3: Neisseria gonorrhoeae causes gonorrhea, not syphilis. Step 4: Chlamydia trachomatis causes chlamydia, not syphilis. Step 5: Escherichia coli is a common gut bacterium and not responsible for syphilis. Treponema pallidum is the correct answer as it is the specific bacterium known to cause syphilis.

Correct Answer: A

Rationale: The correct answer is A: Amebiasis. Amebiasis is caused by the parasite Entamoeba histolytica, presenting with symptoms like acute abdominal pain, bloody diarrhea with mucus, and inconstant shape organisms seen in fecal smear. The presence of erythrocytes supports the diagnosis due to the parasite's ability to invade intestinal mucosa causing bleeding. Choice B (Lambliasis) presents with different symptoms like steatorrhea and fatigue. Choice C (Schistosomiasis) is mainly associated with liver and urinary symptoms. Choice D (Intestinal trichomoniasis) commonly presents with watery diarrhea and does not typically cause bloody stools. Therefore, based on the patient's symptoms and fecal smear findings, Amebiasis is the most likely diagnosis.