Correct Answer: B

Rationale: The correct answer is B: Gram's staining method. The rationale for this is as follows: Gentian violet and Lugol's solution are used as primary and mordant stains in the Gram's staining method. Spiritus 960 is used as a decolorizer, and water fuchsin is used as a counterstain. This staining method is commonly used in microbiology to differentiate bacteria into Gram-positive and Gram-negative based on their cell wall characteristics. A: Leffler's staining method is used for the identification of diphtheria bacilli. C: Ziehl-Neelsen's staining method is used for acid-fast bacteria like Mycobacterium tuberculosis. D: Neisser's staining method is used for the identification of Neisseria species. In summary, the use of specific reactives and stainers in the question indicates the application of the Gram's staining method for bacterial differentiation.

Correct Answer: D

Rationale: Rationale: 1. Mycobacterium tuberculosis, Legionella pneumophila, Brucella melitensis can evade complete phagocytosis. 2. Mycobacterium tuberculosis inhibits phagosome-lysosome fusion. 3. Legionella pneumophila can prevent phagosome maturation. 4. Brucella melitensis can survive and replicate within phagocytes. 5. A + B + C together represent non-complete phagocytosis. Summary: - A, B, C individually cause incomplete phagocytosis. - Only when all three are present together (D) is non-complete phagocytosis observed.

Correct Answer: C

Rationale: The correct answer is C: adhesion to surfaces and conjugation. Pili are hair-like structures on the surface of bacteria that help them adhere to surfaces and facilitate the transfer of genetic material during conjugation. This is essential for bacterial survival and reproduction. Choice A is incorrect because protection from desiccation is typically associated with other structures like capsules. Choice B is incorrect as motility in bacteria is usually facilitated by flagella. Choice D is incorrect as spore formation involves the formation of specialized structures, not pili.

Correct Answer: A

Rationale: The correct answer is A: Staphylococcus epidermidis. The presence of Gram-positive cocci in clusters, along with being catalase-positive and coagulase-negative, is indicative of Staphylococcus species. Staphylococcus epidermidis is commonly found on the skin, making it likely to cause wound infections. Summary: B: Staphylococcus aureus is catalase-positive and coagulase-positive. C: Streptococcus pyogenes is catalase-negative. D: Enterococcus faecalis is catalase-negative.

Correct Answer: A

Rationale: The correct answer is A: Parvoviridae. Parvoviruses are DNA viruses, as their genetic material is composed of DNA. This distinguishes them from RNA viruses like Picornaviridae, Astroviridae, and Togaviridae. Parvoviruses replicate in the host cell nucleus, further confirming their DNA nature. Therefore, based on their DNA composition and replication process, Parvoviridae is the correct choice.