During the staining of sputum smear of a patient with suspected croupous pneumonia the following reactives and stainers were used: gentian violet solution, Lugol's solution, 960 spiritus and water fuchsin. What method of staining is used in this case?

Correct Answer: B

Rationale: The correct answer is B: Gram's staining method. The rationale for this is as follows: Gentian violet and Lugol's solution are used as primary and mordant stains in the Gram's staining method. Spiritus 960 is used as a decolorizer, and water fuchsin is used as a counterstain. This staining method is commonly used in microbiology to differentiate bacteria into Gram-positive and Gram-negative based on their cell wall characteristics. A: Leffler's staining method is used for the identification of diphtheria bacilli. C: Ziehl-Neelsen's staining method is used for acid-fast bacteria like Mycobacterium tuberculosis. D: Neisser's staining method is used for the identification of Neisseria species. In summary, the use of specific reactives and stainers in the question indicates the application of the Gram's staining method for bacterial differentiation.

Correct Answer: D

Rationale: The correct answer is D: reemerging diseases. Group II category diseases by the NIAID are those that have reemerged or have the potential to reemerge. This classification helps prioritize research and resources. Choice A is incorrect because new foodborne pathogens fall under different categories. Choice B is incorrect as eradicated diseases are not part of the reemerging group. Choice C is incorrect because bioterrorism agents are classified separately. Therefore, the correct answer is D as it aligns with the purpose of Group II category diseases.

Correct Answer: D

Rationale: The correct answer is D: endemic. Endemic refers to a disease that is consistently present in a particular population or region at a relatively stable level. This means the disease is always present in that specific area. Pandemic (A) refers to a disease that spreads across multiple countries or continents. Epidemic (B) refers to a sudden increase in the number of cases of a disease above what is normally expected in that population. Sporadic (C) refers to occasional cases of a disease occurring irregularly in a population. Therefore, the correct choice is D as it specifically describes a disease that is generally present in a given population.

Correct Answer: A

Rationale: Pili in bacteria are responsible for conjugation, allowing the transfer of genetic material between cells. They form a bridge between donor and recipient cells, facilitating the exchange of DNA. Flagella are for movement, capsules for protection, and ribosomes for protein synthesis. Pili is the only structure directly involved in genetic material exchange.

Correct Answer: A

Rationale: The correct answer is A: Streptococcus pyogenes. This bacterium is the most likely causative agent due to its characteristic features. Streptococcus pyogenes is Gram-positive cocci in chains, which matches the description provided in the question. It is also known to be beta-hemolytic, causing complete lysis of red blood cells on blood agar. Streptococcus pyogenes is a common pathogen causing sore throat, often associated with pharyngitis. Explanation for incorrect choices: B: Streptococcus pneumoniae - This bacterium is Gram-positive but usually occurs in pairs (diplococci) and is alpha-hemolytic. C: Enterococcus faecalis - This bacterium is Gram-positive cocci but is not typically beta-hemolytic and is not commonly associated with sore throat infections. D: Staphylococcus aureus - This bacterium is Gram-positive cocci but usually occurs in clusters and

Correct Answer: D

Rationale: Certainly! The correct answer is D: Pertussis exotoxin. Pertussis toxin primarily affects the respiratory system causing whooping cough. It does not directly target the nervous system like the other toxins listed. Tetanospasmin causes muscle spasms, Diphtheria exotoxin affects the heart and nerves, and Botulinum exotoxin causes paralysis by blocking neurotransmitter release. Therefore, Pertussis exotoxin is the only toxin among the options that does not manifest with neurological symptoms.

Correct Answer: A

Rationale: Rationale: 1. Vaginitis is a common bacterial infection in the female reproductive system. 2. Toxic shock syndrome can be caused by certain bacterial infections. 3. Endometritis is inflammation of the endometrium often due to bacterial infections. 4. Salpingitis is inflammation of the fallopian tubes, commonly caused by bacteria. Summary: - Choice B: Nephritis is a kidney infection, not related to female reproductive system infections. - Choice C: Nephritis and cystitis are not specific to the female reproductive system. - Choice D: Cystitis is a bladder infection, not typically considered part of female reproductive system infections.

Correct Answer: A

Rationale: Rationale: 1. Salmonella typhi causes typhoid fever, which can lead to intestinal infections. 2. Shigella flexneri causes dysentery, primarily affecting the colon. 3. Helicobacter pylori causes stomach ulcers, not intestine infections. Summary: A is correct as Salmonella typhi causes intestine infection. B and C are incorrect as they cause different gastrointestinal issues. D is incorrect as A is a valid choice.

Correct Answer: A

Rationale: The correct answer is A: Pseudomonas aeruginosa. Pseudomonas aeruginosa is known for producing a bluish-green pigment called pyocyanin, which presents as a cyan pigment. This bacterium is commonly associated with wound infections and is often found in oral surgery complications. Proteus vulgaris (B) and Klebsiella pneumoniae (D) are not typically associated with cyan pigment production, making them less likely causative agents. Bacillus subtilis (C) is not known to produce cyan pigment and is not a common pathogen in wound infections. Therefore, based on the presence of cyan pigment and the clinical context of an oral surgery complication, Pseudomonas aeruginosa is the most probable causative agent.