During the staining of sputum smear of a patient with suspected croupous pneumonia the following reactives and stainers were used: gentian violet solution, Lugol's solution, 960 spiritus and water fuchsin. What method of staining is used in this case?

Correct Answer: A

Rationale: The correct answer is A: Leffler's method. In this staining method, gentian violet solution is used as the primary stain, followed by Lugol's solution as a mordant, 960 spiritus as a decolorizer, and water fuchsin as a counterstain. Gentian violet stains the organisms purple, Lugol's solution helps in fixing the dye, 960 spiritus decolorizes non-specifically, and water fuchsin acts as a counterstain to help visualize the bacteria. Leffler's method is specifically used for staining sputum smears in suspected cases of croupous pneumonia. Summary: B: Gram's staining uses crystal violet, iodine, alcohol, and safranin. It is mainly used for distinguishing between Gram-positive and Gram-negative bacteria. C: Ziehl-Neelsen's staining is used for acid-fast bacteria like Mycobacterium tuberculosis using carbol fuchsin and acid-alcohol

Correct Answer: C

Rationale: The correct answer is C: Leishmaniasis. The description of thin microorganisms with 12-14 uniform tendrils, 10-13 micrometers in length, and pale pink color matches the characteristics of Leishmania parasites seen in Giemsa-stained samples. Leishmania parasites are known to have a distinctive appearance with pointed tips and a specific size range. Syphilis (choice A) is caused by Treponema pallidum, which appears as tightly coiled spirochetes, not tendrils. Trypanosomiasis (choice B) is caused by Trypanosoma parasites, which have a different morphology and staining pattern. Relapsing fever (choice D) is caused by Borrelia recurrentis, which does not match the description given. Therefore, the correct choice is C as the description aligns closely with the characteristic features of Leishmania parasites.

Correct Answer: D

Rationale: The correct answer is D: Agglutination test type Gruber. This is because the Gruber agglutination test is not typically used for the serological identification of Yersinia pestis. The other options (A, B, C) are commonly used methods for serological identification of Y. pestis: A) Precipitation reaction involves the formation of a visible precipitate when Y. pestis antigen reacts with specific antibodies. B) Indirect hemagglutination test detects antibodies against Y. pestis by measuring the agglutination of red blood cells. C) Immunofluorescence test uses fluorescently labeled antibodies to detect specific antigens of Y. pestis. In summary, the Gruber agglutination test is not a standard method for serological identification of Yersinia pestis, unlike the other options provided.

Correct Answer: D

Rationale: The correct answer is D because all three bacteria listed produce toxins that cause damage to host tissues. Clostridium tetani produces tetanospasmin toxin causing tetanus, Vibrio cholerae produces cholera toxin leading to severe diarrhea, and Staphylococcus aureus releases toxins causing tissue damage in various infections. Therefore, all of the above choices are correct in terms of producing toxins that harm host tissues.

Correct Answer: C

Rationale: The correct answer is C: All microorganisms. Sterilization aims to eliminate all microorganisms, including pathogenic and non-pathogenic ones, to ensure complete absence of viable organisms. This is crucial in medical, laboratory, and food preparation settings to prevent contamination and spread of infections. Choices A and B are incorrect because sterilization does not differentiate between pathogenic and non-pathogenic microorganisms, aiming to eradicate all. Choice D is incorrect as sterilization does involve releasing subjects (microorganisms) using physical methods.

Correct Answer: B

Rationale: Bacillus cereus is known to cause food poisoning due to the production of toxins in contaminated food. It is not the causative agent of anthrax or endocarditis. The correct answer is B because Bacillus cereus is commonly associated with foodborne illnesses, leading to symptoms such as nausea, vomiting, and diarrhea. It's essential to understand the specific characteristics and pathogenicity of Bacillus cereus to differentiate it from other bacterial infections.

Correct Answer: A

Rationale: The correct answer is A: Candida fungi. Candida fungi are known to cause oral thrush, characterized by whitish spots on the oral mucosa. The presence of gram-positive oval budding cells in the smear preparations is indicative of Candida species. Staphylococci (choice B) are gram-positive cocci, not oval budding cells. Sarcinae (choice C) are gram-positive bacteria that form cuboidal packets, not oval budding cells. Actinomycetes (choice D) are filamentous gram-positive bacteria, not oval budding cells like Candida fungi.

Correct Answer: B

Rationale: The correct answer is B: Staphylococcus aureus. This bacteria is known to cause skin infections and toxic shock syndrome due to its ability to produce toxins that can lead to severe symptoms. Staphylococcus aureus is commonly found on the skin and can enter the body through cuts or wounds, causing infections. Other choices are incorrect because Pseudomonas aeruginosa typically causes infections in immunocompromised individuals, Micrococcus luteus is a common skin bacterium that is not typically pathogenic, and Propionibacterium acnes is associated with acne, not skin infections or toxic shock syndrome.

Correct Answer: A

Rationale: The correct answer is A: Bacteria. Urinary tract infections (UTIs) are primarily caused by the invasion of bacteria into the urinary tract. Bacteria, especially Escherichia coli (E. coli), are the most common culprits. They enter the urinary tract through the urethra and can multiply in the bladder, leading to infection. Viruses (choice B) and fungi (choice C) are not typical causes of UTIs. "None of the above" (choice D) is incorrect as bacteria are indeed the main cause of UTIs.