Correct Answer: C

Rationale: The correct answer is C (coli index of 250 and coli titer of 4). The coli index is calculated by multiplying the number of colonies by the reciprocal of the volume filtered, which gives 2 colonies x (1/0.5 L) = 4. The coli titer is obtained by dividing the volume filtered by the number of colonies, which gives 0.5 L / 2 colonies = 0.25 L or 250 ml. This indicates the concentration of coliform bacteria in the water sample. Choice A (4 and 250) is incorrect because it has the correct coli index but the values are reversed for coli index and coli titer. Choice B (2 and 500) is incorrect as it provides the correct coli titer value but the values are reversed for coli index and coli titer. Choice D (500 and 2) is incorrect because it reverses the values for both coli index and coli titer.

Correct Answer: A

Rationale: Step-by-step rationale for choice A being correct: 1. Penicillin allergy involves IgE antibodies binding to penicillin. 2. This triggers mast cell degranulation, releasing histamine. 3. Histamine causes allergic symptoms like rash, swelling. 4. This immune response characterizes Type I hypersensitivity reactions. Summary of other choices: B: Type II reactions involve antibody-mediated cell destruction, not seen in penicillin allergy. C: Type III reactions involve immune complex deposition, not typical in penicillin allergy. D: Type IV reactions are delayed hypersensitivity reactions, not immediate like penicillin allergy.

Correct Answer: A

Rationale: The correct answer is A: Staphylococcus aureus. Staphylococcus aureus is a Gram-positive cocci bacterium that forms clusters and commonly causes pneumonia with a productive cough and fever. It is frequently found in sputum smears in such cases. Streptococcus pneumoniae (B) is another common cause of pneumonia but typically appears in pairs or chains, not clusters. Neisseria meningitidis (C) is a Gram-negative diplococcus that causes meningitis, not pneumonia. Klebsiella pneumoniae (D) is a Gram-negative bacterium that can cause pneumonia but appears as Gram-negative rods, not Gram-positive cocci in clusters.

Correct Answer: B

Rationale: The correct answer is B: Outer membrane containing lipopolysaccharides. Gram-negative bacteria have an outer membrane that contains lipopolysaccharides, which are important for protection and pathogenicity. This outer membrane is a unique feature of Gram-negative bacteria and is not present in Gram-positive bacteria, making it a key characteristic. A: Incorrect because Gram-negative bacteria have a thin peptidoglycan layer compared to Gram-positive bacteria. C: Incorrect because teichoic acids are a characteristic of Gram-positive bacteria, not Gram-negative bacteria. D: Incorrect because all bacteria, including Gram-negative bacteria, have a cell wall.

Correct Answer: A

Rationale: Step-by-step rationale: 1. The presence of a nucleus with a nuclear membrane indicates eukaryotic cells. 2. Eukaryotes have genetic material in chromosomes made of DNA and proteins. 3. Mitotic division is characteristic of eukaryotic cells. 4. Therefore, the organisms described are eukaryotes. Summary: - Bacteriophages (B) are viruses that infect bacteria. - Prokaryotes (C) lack a nucleus and their genetic material is not organized into chromosomes. - Viruses (D) are not considered living organisms and do not have a nucleus or chromosomes.