During an outbreak of cholera, a fecal smear revealed Gram-negative, comma-shaped, motile bacteria. What staining method would best confirm the diagnosis?

Correct Answer: A

Rationale: The correct answer is A: Gram staining. This method is used to differentiate bacteria based on their cell wall composition. In cholera, the causative agent is Vibrio cholerae, which is Gram-negative. By performing Gram staining, the bacteria will appear pink/red under the microscope, confirming the presence of Gram-negative bacteria. Choice B: Neisser's staining is used for staining capsules of bacteria, not suitable for confirming cholera diagnosis. Choice C: Ziehl-Neelsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for confirming cholera diagnosis. Choice D: Burri-Gins staining is not a standard staining method in microbiology and is not relevant for confirming cholera diagnosis.

Correct Answer: B

Rationale: The correct answer is B: Motility. The bacterial flagellum is a whip-like appendage that helps bacteria move by rotating like a propeller. This motility allows bacteria to navigate their environment, find nutrients, and avoid harmful conditions. Choices A, C, and D are incorrect because the bacterial flagellum is not involved in protein synthesis, DNA replication, or spore formation. Protein synthesis occurs in ribosomes, DNA replication takes place in the nucleus or nucleoid, and spore formation is a specialized process involving the production of spores for survival in harsh conditions.

Correct Answer: B

Rationale: Fimbriae in bacteria are hair-like appendages that help in adhesion to surfaces. This is essential for colonization and infection. Fimbriae do not provide motility (flagella do), facilitate protein synthesis (ribosomes do), or help in endospore formation (specialized structures do). Adhesion is the primary function of fimbriae, allowing bacteria to stick to host cells or abiotic surfaces.

Correct Answer: A

Rationale: Aminoglycosides primarily inhibit protein synthesis by binding to the 30S ribosomal subunit in bacteria. This action leads to misreading of mRNA and ultimately disrupts the translation process, resulting in faulty protein production and bacterial cell death. Incorrect choices: B: Aminoglycosides do not inhibit DNA replication. C: Aminoglycosides do not disrupt cell membrane integrity. D: Aminoglycosides do not inhibit folic acid synthesis. In summary, the correct choice (A) aligns with the known mechanism of action of aminoglycosides, making it the most appropriate answer.

Correct Answer: A

Rationale: The correct answer is A: Nitrogen-fixing bacteria. These bacteria can convert atmospheric nitrogen into a form that plants can use, promoting plant growth. Denitrifying bacteria (B) convert nitrates back into atmospheric nitrogen. Photosynthetic bacteria (C) use photosynthesis to produce energy and don't fix nitrogen. Fermentative bacteria (D) break down organic compounds without fixing nitrogen. In summary, only nitrogen-fixing bacteria (A) play a crucial role in the nitrogen cycle by converting atmospheric nitrogen into a usable form for plants.