Differential growth media are:

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microbiology an introduction 13th edition test bank Questions

Question 1 of 5

Differential growth media are:

Correct Answer: A

Rationale: The correct answer is A because differential growth media, such as Endo agar and MacConkey agar, contain specific indicators or substrates that allow for the differentiation of microorganisms based on their metabolic properties. For example, Eosin methylene blue agar differentiates between lactose fermenting and non-fermenting bacteria based on color changes. Levine's formulation is selective for Gram-negative bacteria due to bile salts and crystal violet. In contrast, the other choices do not have specific indicators for differentiation among microorganisms. Lowenstein-Jensen and SS agar are selective media for mycobacteria and Salmonella/Shigella species, respectively. ACA and BPLS agar are selective but not differential, and Nutrient agar and Peptone water are general-purpose media without differential properties.

Question 2 of 5

A 70-year-old man was admitted to the hospital after suffering extensive third-degree burns. Three days later he spiked a fever and there was pus on the dressing that had blue-green colour. Gram stain of the pus revealed gram-negative rods. The most possible cause of these findings is:

Correct Answer: A

Rationale: The correct answer is A: The pyocyanin released by Pseudomonas aeruginosa. Pyocyanin is a blue-green pigment produced by Pseudomonas aeruginosa, a common cause of nosocomial infections in burn patients. The presence of blue-green pus and gram-negative rods in the wound along with fever suggests an infection with Pseudomonas aeruginosa. The other choices are incorrect because E. Coli typically causes gastrointestinal infections and releases enterotoxins, Staphylococcus aureus releases hemolysins causing hemolysis of red blood cells, and Shigella dysenteriae releases shigatoxin causing bloody diarrhea, none of which match the clinical presentation described in the question.

Question 3 of 5

The most common urinary tract pathogen is:

Correct Answer: C

Rationale: The correct answer is C: Escherichia coli. E.coli is the most common urinary tract pathogen due to its ability to adhere to and infect the urinary tract epithelium. It accounts for approximately 80-85% of urinary tract infections. It possesses specific virulence factors that enable it to colonize and cause infection in the urinary tract. Proteus mirabilis (A) is known for causing complicated UTIs, but it is less common than E.coli. Staphylococcus aureus (B) is not a common urinary pathogen. Pseudomonas aeruginosa (D) is more commonly associated with hospital-acquired infections, rather than community-acquired urinary tract infections.

Question 4 of 5

The one-way transfer of genetic material from a donor cell to a recipient cell by direct cell to cell contact is:

Correct Answer: B

Rationale: The correct answer is B: Conjugation. Conjugation involves the transfer of genetic material from a donor cell to a recipient cell through direct cell-to-cell contact, typically mediated by a conjugative plasmid. During conjugation, a conjugative pilus forms a bridge between the donor and recipient cells, allowing the transfer of genetic material. A: Replication is the process of copying DNA to produce two identical DNA molecules. It does not involve the transfer of genetic material between cells. C: Transformation is the uptake and incorporation of foreign DNA from the environment by a bacterial cell. It does not require direct cell-to-cell contact. D: Transduction is the transfer of genetic material from one bacterium to another by a bacteriophage vector. It does not involve direct cell-to-cell contact between the donor and recipient cells.

Question 5 of 5

The complex invaginations of the prokaryotic cytoplasmic membrane are called:

Correct Answer: D

Rationale: The correct answer is D: Mesosomes. Mesosomes are infoldings of the prokaryotic cytoplasmic membrane that play a role in cell division and respiration. This structure is not found in eukaryotic cells. Cristae (A) are infoldings of the mitochondrial inner membrane in eukaryotic cells. Granum (B) refers to stacks of thylakoid membranes in chloroplasts. Flagella (C) are whip-like structures used for movement and are not related to cytoplasmic membrane invaginations. Therefore, the correct answer is D as mesosomes are unique to prokaryotic cells and are involved in various cellular processes.

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