Correct Answer: A

Rationale: Step 1: Chlorhexidine belongs to the group of biguanides, making choice A accurate. Step 2: It is commonly used for disinfection of hands, skin, and mucous membranes. Step 3: It has a broad spectrum of antimicrobial activity, making it widely applicable. Summary: Choice B is incorrect as chlorhexidine is not classified as an alcohol. Choice C is incorrect as chlorhexidine has various applications. Choice D is incorrect as chlorhexidine is generally safe for use with low toxicity.

Correct Answer: A

Rationale: The correct answer is A: Gram staining. This method is used to differentiate bacteria based on their cell wall composition. In cholera, the causative agent is Vibrio cholerae, which is Gram-negative. By performing Gram staining, the bacteria will appear pink/red under the microscope, confirming the presence of Gram-negative bacteria. Choice B: Neisser's staining is used for staining capsules of bacteria, not suitable for confirming cholera diagnosis. Choice C: Ziehl-Neelsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for confirming cholera diagnosis. Choice D: Burri-Gins staining is not a standard staining method in microbiology and is not relevant for confirming cholera diagnosis.

Correct Answer: D

Rationale: The correct answer is D: BSL4. Ebola viruses are highly infectious and pose a high risk to laboratory workers. BSL4 facilities are designed to handle agents that pose a severe threat to human health and have no available vaccines or treatments. These facilities have strict guidelines for handling infectious agents, including full-body suits, negative air pressure, and decontamination processes to prevent any possible exposure. BSL1, BSL2, and BSL3 are not adequate for handling Ebola viruses due to their lower levels of containment and safety measures.

Correct Answer: B

Rationale: The correct answer is B: Staphylococcus aureus. This bacteria is known to cause skin infections and toxic shock syndrome due to its ability to produce toxins that can lead to severe symptoms. Staphylococcus aureus is commonly found on the skin and can enter the body through cuts or wounds, causing infections. Other choices are incorrect because Pseudomonas aeruginosa typically causes infections in immunocompromised individuals, Micrococcus luteus is a common skin bacterium that is not typically pathogenic, and Propionibacterium acnes is associated with acne, not skin infections or toxic shock syndrome.

Correct Answer: A

Rationale: Rationale: A is incorrect because whooping cough resurgence is mainly due to decreased vaccine immunity, not changing antigenicity. B is correct as P. aeruginosa can indeed cause infections with blue-green pus due to its pigment production. C is correct as H. influenzae type b capsule is a virulence factor contributing to invasive disease. D is correct as Legionella infection is acquired via inhalation of contaminated water aerosols. Therefore, A is incorrect as the rise in whooping cough is not due to changing antigenicity but vaccine immunity decline.