Cells that contain a nucleus are __________ cells.

Correct Answer: B

Rationale: The correct answer is B: eukaryotic. Eukaryotic cells have a well-defined nucleus that encloses the genetic material. The nucleus is a distinguishing feature of eukaryotic cells, separating them from prokaryotic cells (choice A) which lack a nucleus. Anucleate cells (choice C) are cells that do not have a nucleus at all. Haploid cells (choice D) contain a single set of chromosomes, but this does not necessarily imply the presence of a nucleus. Therefore, the presence of a nucleus is what categorizes cells as eukaryotic.

Correct Answer: A

Rationale: The correct answer is A: Clostridium botulinum. Clostridium botulinum produces the botulinum toxin, which causes botulism. This bacteria thrives in anaerobic environments and can contaminate improperly canned foods. Clostridium tetani causes tetanus, not botulism. Staphylococcus aureus produces enterotoxins causing food poisoning, not botulism. Escherichia coli can cause food poisoning through various toxins, but not botulism. Therefore, the correct choice is Clostridium botulinum as it specifically produces the botulinum toxin responsible for botulism.

Correct Answer: A

Rationale: Step-by-step rationale: 1. Gram-positive rods with terminal spores suggest Clostridium genus. 2. Terminal spores are characteristic of Clostridium perfringens. 3. C. perfringens is known to cause wound infections. 4. Therefore, the most likely causative agent is Clostridium perfringens. Summary: A: Clostridium perfringens is correct due to its characteristic features and association with wound infections. B: Bacillus anthracis does not typically form terminal spores. C: Clostridium tetani causes tetanus, not wound infections. D: Bacillus cereus does not produce terminal spores like Clostridium perfringens.

Correct Answer: A

Rationale: The correct answer is A: Neisser staining method. Neisser staining method is used to stain diphtheria bacilli, which appear as blue rods with thickenings at the poles. The rationale is that Neisser staining specifically targets and highlights the characteristics of the diphtheria bacilli. Incorrect choices: B: Gram staining is not specific for diphtheria bacilli and does not show the characteristic blue rods with thickenings at the poles. C: Ziehl-Neelsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for diphtheria bacilli. D: Loeffler staining is used for diphtheria bacilli but does not produce blue rods with thickenings at the poles as seen in this case.

Correct Answer: C

Rationale: The correct answer is C: Method of Moller. This method uses malachite green to stain bacterial spores due to their resistance to decolorization. The other choices, A, B, and D, are incorrect because Romanovski-Gymsa is used for staining blood smears, Ziehl-Nielsen is used for acid-fast bacteria, and Neisser is used for staining Neisseria species, not bacterial spores.

Correct Answer: A

Rationale: The correct answer is A: Clostridium perfringens. Clostridium perfringens is known to cause gas gangrene and is a Gram-positive rod that produces spores and gas in tissue infections. Clostridium tetani causes tetanus, not gas gangrene. Bacillus anthracis causes anthrax, not gas gangrene. Staphylococcus aureus is a Gram-positive cocci and does not produce spores or gas in the context of gas gangrene. Therefore, based on the characteristics of the microorganism isolated from the wound sample, Clostridium perfringens is the most likely causative agent.

Correct Answer: D

Rationale: The correct answer is D: Hypersensitivity of delayed type. The symptoms described occur 5-10 days after exposure to the antigen, consistent with a delayed hypersensitivity reaction. This type of reaction involves T cells and is characterized by cell-mediated immune response, leading to inflammation and tissue damage. Choices A, B, and C are incorrect because they are associated with different mechanisms of allergic reactions. Immunocomplex reactions (choice A) involve antigen-antibody complexes depositing in tissues, immediate hypersensitivity reactions (choice B) involve IgE-mediated mast cell degranulation, and cytotoxic reactions (choice C) involve antibodies targeting specific cells for destruction.

Correct Answer: C

Rationale: The correct answer is C because Rickettsia requires living cells for growth and replication. Chick embryos, cell cultures, and lab animals provide a suitable environment for Rickettsia isolation. Skin-allergy tests (A) and serological methods for proving antibodies (B) are not directly used for isolating Rickettsia as they do not support the growth of the bacteria. "None of the above" (D) is incorrect as option C involves the appropriate methods for isolating Rickettsia.

Correct Answer: A

Rationale: The correct answer is A: Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative rod that is a lactose non-fermenter commonly associated with deep tissue infections. It is an opportunistic pathogen known for its ability to cause infections in immunocompromised individuals. P. aeruginosa is often resistant to multiple antibiotics and can form biofilms, making it difficult to treat. Summary of why other choices are incorrect: B: Escherichia coli is a Gram-negative rod that ferments lactose and is commonly found in the gastrointestinal tract. It is not typically associated with deep tissue infections. C: Salmonella typhi is a Gram-negative rod that causes typhoid fever, primarily affecting the gastrointestinal system, not deep tissue infections. D: Shigella dysenteriae is a Gram-negative rod that causes bacillary dysentery, which primarily affects the gastrointestinal tract and does not typically cause deep tissue infections.