ATI RN
microbiology an evolving science test bank Questions
Question 1 of 5
Capsuliferous bacteria has been detected during microbiological inspection of crude drugs. What method of staining has been used to detect capsules?
Correct Answer: A
Rationale: The correct answer is A: Burri-Gins. This staining method specifically targets the detection of capsules in bacteria. It involves staining the capsules with crystal violet and the background with safranin. This staining technique helps visualize the capsules as a distinct halo around the bacteria. Summary: - Choice B (Ziehl-Neelsen) is used for acid-fast staining to detect Mycobacterium species. - Choice C (Neisser) is used for staining Neisseria species. - Choice D (Gram) is a common staining method used to differentiate bacteria based on cell wall composition (Gram-positive and Gram-negative), but it does not specifically target capsules.
Question 2 of 5
Botulism is a disease caused by the production of a potent neurotoxin by bacteria of the genus:
Correct Answer: D
Rationale: The correct answer is D: Clostridium. Clostridium bacteria produce the neurotoxin responsible for botulism. Clostridium botulinum is specifically known for causing this disease. Staphylococcus (A), Klebsiella (B), and Salmonella (C) do not produce the neurotoxin associated with botulism. Staphylococcus typically causes skin infections, Klebsiella is often associated with pneumonia and urinary tract infections, and Salmonella is known for causing food poisoning. Therefore, the correct answer is Clostridium due to its specific association with botulism.
Question 3 of 5
The relations between the macroorganism and the resident skin microflora are defined as:
Correct Answer: A
Rationale: The correct answer is A: Commensalism. In this relationship, the skin microflora benefits from the host without causing harm or benefitting it. This is evident in the mutualistic interaction where the microflora receive nutrients and a place to live, while the host remains unaffected. Choice B, Infection, is incorrect because it implies harm to the host. Choice C, Parasitism, is also incorrect as it involves one organism benefiting at the expense of the other. Choice D, No correct answer, is not applicable here as Commensalism accurately describes the relationship between the macroorganism and skin microflora.
Question 4 of 5
Which of the following is true about plasmids?
Correct Answer: C
Rationale: Plasmids often carry antibiotic resistance genes, making choice C correct. Plasmids are circular DNA structures, not linear (A). They are found in both gram-positive and gram-negative bacteria, not exclusively in gram-positive bacteria (B). Plasmids are separate from the bacterial chromosome, not part of it (D). Therefore, choice C is the correct answer.
Question 5 of 5
A patient with acute food poisoning had fecal cultures grown on Endo agar, resulting in colorless colonies. What microorganism is most likely responsible?
Correct Answer: A
Rationale: Step 1: Endo agar is a selective and differential media commonly used to isolate gram-negative bacteria. Step 2: Colorless colonies on Endo agar indicate the absence of lactose fermentation. Step 3: Salmonella is a gram-negative bacterium that does not ferment lactose, leading to colorless colonies on Endo agar. Step 4: Therefore, the correct answer is A: Salmonella. Summary: B: Escherichia coli ferments lactose, producing pink colonies on Endo agar. C: Staphylococcus is a gram-positive bacterium and would not grow on Endo agar. D: Shigella is a gram-negative bacterium but ferments lactose, resulting in pink colonies on Endo agar.
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