Correct Answer: D

Rationale: The correct answer is D (15-Oct) because boiling items for at least 15 minutes is necessary to effectively kill most vegetative bacteria and viruses. This duration ensures that the water reaches a high enough temperature to destroy harmful microorganisms. Choices A, B, and C are incorrect because they do not provide a sufficient amount of time for the water to reach the necessary temperature to effectively kill bacteria and viruses. Shorter durations may not be effective in ensuring food safety.

Correct Answer: C

Rationale: The correct answer is C because diphtheria exotoxin is absorbed into the underlying healthy tissues and spreads throughout the body. This is due to the toxin's ability to enter the bloodstream and target various organs, causing systemic effects. Choice A is incorrect as the toxin does not stay localized at the site of infection. Choice B is incorrect as diphtheria exotoxin does not directly affect neuro-muscular transmission. Choice D is also incorrect as the toxin does spread throughout the body.

Correct Answer: A

Rationale: The correct answer is A: Entamoeba gingivalis. This organism matches the description provided in the question - unicellular with pseudopodia and ingested white blood cells, commonly found in dental plaque. Entamoeba gingivalis is known to inhabit the oral cavity and is associated with periodontal disease. Trichomonas vaginalis, Giardia lamblia, and Balantidium coli do not match the characteristics mentioned in the question and are not typically found in the oral cavity.

Correct Answer: C

Rationale: The correct answer is C: Negative stain. Negative staining uses acidic dyes which are repelled by the bacterial cell wall components, highlighting bacteria with a large amount of peptidoglycan. Acid-fast stain (A) is used to identify Mycobacterium species with waxy cell walls. Gram stain (B) differentiates bacteria into Gram-positive and Gram-negative based on cell wall composition. Option D is a duplicate of option C and is incorrect.

Correct Answer: D

Rationale: The correct answer is D because enveloped viruses have a lipid bilayer envelope derived from the host cell membrane, which is a complex structure, not exhibiting any specific symmetry. Choice A (cubic symmetry) is incorrect as enveloped viruses do not have any specific geometric shape. Choice B (spiral symmetry) is incorrect as this type of symmetry is typically seen in non-enveloped viruses like helical viruses. Choice C (complex symmetry) is also incorrect as enveloped viruses do not exhibit a specific symmetrical pattern. Therefore, the correct answer is D because enveloped viruses do not have a defined symmetry and can vary in shape and structure.