Bacteria capable of growing at low temperatures are called:

Correct Answer: C

Rationale: The correct answer is C: psychrophiles. Psychrophiles are bacteria that can grow at low temperatures, typically below 20°C. This is because they have adapted to survive and thrive in cold environments. Mesophiles (A) grow best at moderate temperatures, thermophiles (B) thrive in high temperatures, and halophiles (D) prefer high salt concentrations. Psychrophiles are specifically adapted to cold temperatures, making them the correct choice in this scenario.

Correct Answer: B

Rationale: The correct answer is B because retroviruses, including those in the Family Retroviridae, have a capsid consisting of two copies of single-stranded RNA (ssRNA). This is a defining characteristic of retroviruses, which use reverse transcriptase to convert their ssRNA into DNA. This DNA is then integrated into the host cell's genome. Choices A, C, and D are incorrect because Family Retroviridae viruses are not helical non-enveloped viruses (C), they are not helical enveloped viruses (A), and they do not have a complex type of symmetry (D).

Correct Answer: B

Rationale: The correct answer is B: Gram's staining method. The rationale for this is as follows: Gentian violet and Lugol's solution are used as primary and mordant stains in the Gram's staining method. Spiritus 960 is used as a decolorizer, and water fuchsin is used as a counterstain. This staining method is commonly used in microbiology to differentiate bacteria into Gram-positive and Gram-negative based on their cell wall characteristics. A: Leffler's staining method is used for the identification of diphtheria bacilli. C: Ziehl-Neelsen's staining method is used for acid-fast bacteria like Mycobacterium tuberculosis. D: Neisser's staining method is used for the identification of Neisseria species. In summary, the use of specific reactives and stainers in the question indicates the application of the Gram's staining method for bacterial differentiation.

Correct Answer: A

Rationale: The Ziehl-Neelsen stain is specific for acid-fast bacteria like Mycobacterium tuberculosis. The bright red bacilli seen in the smear indicate the presence of acid-fast organisms, which is characteristic of Mycobacterium tuberculosis. Staphylococcus aureus, Clostridium tetani, and Escherichia coli are not acid-fast bacteria and would not be stained red using the Ziehl-Neelsen method. Therefore, the correct answer is A: Mycobacterium tuberculosis.

Correct Answer: C

Rationale: The correct answer is C: adhesion to surfaces and conjugation. Pili are hair-like structures on the surface of bacteria that help them adhere to surfaces and facilitate the transfer of genetic material during conjugation. This is essential for bacterial survival and reproduction. Choice A is incorrect because protection from desiccation is typically associated with other structures like capsules. Choice B is incorrect as motility in bacteria is usually facilitated by flagella. Choice D is incorrect as spore formation involves the formation of specialized structures, not pili.