At a bacteriological laboratory animal skins are analyzed by means of Ascoli precipitaion test. What is detected if the reaction is positive?

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Microbiology An Introduction 12th Edition Test Bank Questions

Question 1 of 5

At a bacteriological laboratory animal skins are analyzed by means of Ascoli precipitaion test. What is detected if the reaction is positive?

Correct Answer: A

Rationale: The Ascoli precipitation test is specifically used to detect anthrax agent antigens. The test involves mixing a patient's serum with a solution containing anthrax antigens, leading to precipitation if the antigens are present. Therefore, a positive reaction in this test indicates the presence of anthrax agent antigens. The other choices (B: Brucellosis agent, C: Anaerobic infection toxin, D: Plague agent) are incorrect because the Ascoli precipitation test is not designed to detect these specific agents or toxins.

Question 2 of 5

Botulism agent causes severe food toxicoinfection. Point out the most characteristic morphologic feature of botulism agent.

Correct Answer: B

Rationale: The correct answer is B: Thick gram-positive non-sporeforming bacilli. In botulism, the causative agent, Clostridium botulinum, is a thick gram-positive bacilli that does not form spores. This is a key characteristic morphologic feature. A: Gram-positive spore-forming bacilli with subterminal spore - This is incorrect as Clostridium botulinum does not have subterminal spores. C: Gram-positive spore-forming bacilli with terminal spore - Incorrect because Clostridium botulinum does not form spores. D: Thin mobile spore-forming bacilli with central spore - Incorrect because Clostridium botulinum is not thin, mobile, or spore-forming. In summary, the most characteristic morphologic feature of the botulism agent is being thick, gram-positive, and non-sporeforming, making choice B the correct answer.

Question 3 of 5

In 8 days after a surgery the patient developed tetanus. The surgeon suspects this condition to be caused by suture material contaminated by tetanus agent. The material is delivered to a bacteriological laboratory. What nutrient medium is required for primary inoculation of the suture material?

Correct Answer: A

Rationale: The correct answer is A: Kitt-Tarozzi medium. This medium is used for the primary inoculation of suture material suspected of causing tetanus. The rationale is that Kitt-Tarozzi medium contains the necessary nutrients to support the growth of Clostridium tetani, the bacterium responsible for tetanus. This medium is selective and helps in isolating the specific pathogen from the contaminated suture material. Summary of other choices: B: Endo agar is used for the isolation of Gram-negative bacteria from clinical and non-clinical samples. C: Sabouraud agar is used for the isolation of fungi and yeast. D: Egg-yolk salt agar is used for the detection of lipase-producing bacteria, particularly Staphylococcus aureus.

Question 4 of 5

A 32-year-old patient undergoing dental examination was found to have some rash-like lesions resembling secondary syphilis in the oral cavity. The patient was referred for the serological study with the purpose of diagnosis confirmation. In order to detect antibodies in the serum, living Treponema were used as diagnosticum. What serological test was performed?

Correct Answer: B

Rationale: The correct answer is B: Passive hemagglutination. In this test, red blood cells coated with Treponema antigens agglutinate in the presence of patient's serum antibodies. This indicates the presence of antibodies against Treponema, confirming the diagnosis. A: Immobilization test uses live Treponema to detect specific antibodies, but it is not commonly used for syphilis diagnosis. C: Precipitation test is not commonly used for syphilis diagnosis. D: Complement binding test is not specific for syphilis and is used more for autoimmune diseases. Therefore, passive hemagglutination is the most appropriate serological test in this case due to its specificity and relevance to the presentation of the patient.

Question 5 of 5

Carious cavities of a 29-year-old patient contain the parasitic protozoa. It is established that they relate to the Sarcodina class. Specify these single-celled organisms:

Correct Answer: A

Rationale: The correct answer is A: Entamoeba gingivalis. Firstly, it is important to note that Entamoeba gingivalis is commonly found in the oral cavity, specifically in carious cavities. Secondly, Entamoeba gingivalis belongs to the Sarcodina class, which is characterized by their amoeboid movement using pseudopods. This protozoa is associated with oral health issues, particularly in individuals with poor oral hygiene. Now, let's analyze why the other choices are incorrect: B: Entamoeba histolutica is known for causing amoebic dysentery, not commonly associated with oral cavities. C: Entamoeba coli is typically found in the human large intestine, not in oral cavities. D: Amoeba proteus is a free-living amoeba commonly used in laboratory studies and not typically associated with oral cavity infections.

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