An 18-year-old patient has developed candidiasis after the case of pneumonia treated with β- lactam antibiotic. What antimycotic agent should be prescribed?

Correct Answer: B

Rationale: The correct answer is B: Fluconazole. Rationale: 1. Candidiasis is a fungal infection, so an antimycotic agent is needed. 2. β-lactam antibiotics like ampicillin can lead to fungal overgrowth, necessitating an antimycotic. 3. Fluconazole is an antifungal agent effective against Candida species. 4. Streptomycin is an antibiotic, not an antifungal. 5. Phthalylsulfathiazole is an antibacterial sulfonamide, not an antifungal. 6. Ampicillin is an antibiotic, not an antifungal.

Correct Answer: A

Rationale: The correct answer is A. Method of Ziel-Neelsen. This method uses a special stain (carbol fuchsin) to detect acid-fast organisms like Mycobacterium tuberculosis, the causative agent of tuberculosis. The staining procedure involves heating the slide to help penetrate the mycolic acid layer of the bacteria, making them resistant to decolorization by acid-alcohol. The red stained acid-fast bacilli stand out against the blue counterstain. This method is specific for identifying tubercle bacilli in sputum samples. Choice B: Method of Romanowsky-Giemsa is used for staining blood smears and is not suitable for detecting acid-fast bacilli in sputum. Choice C: Method of Gram is used for staining bacteria based on their cell wall composition and is not effective for identifying acid-fast bacilli like Mycobacterium tuberculosis. Choice D: Method of Neisser is not a specific staining method for acid-fast bacilli and is not suitable

Correct Answer: A

Rationale: The correct answer is A: Alexander Fleming. He discovered the antibiotic properties of Penicillium in 1928. Fleming observed that a mold called Penicillium notatum inhibited the growth of bacteria, leading to the discovery of penicillin. Louis Pasteur is known for his work on pasteurization and microbiology, not penicillin. Joseph Lister is known for pioneering antiseptic surgery techniques. Edward Jenner is known for developing the smallpox vaccine, not penicillin. Fleming's discovery revolutionized medicine by introducing the first antibiotic, penicillin, which has saved countless lives.

Correct Answer: C

Rationale: The correct answer is C: ensure survival under harsh conditions. Bacterial endospores are dormant, resistant structures that form under adverse conditions to protect the bacterial DNA and allow the bacterium to survive extreme environments. This function is crucial for the bacterium's long-term survival. Choice A is incorrect as endospores do not directly produce exotoxins. Choice B is incorrect as endospores do not store nutrients but rather protect the bacterium's genetic material. Choice D is incorrect as endospores do not enhance motility but rather enable survival during unfavorable conditions.

Correct Answer: A

Rationale: Rationale: Bacillus cereus is associated with food intoxication due to its ability to produce toxins that cause gastrointestinal symptoms. B. subtilis is not known to cause food intoxication. B. anthracis causes anthrax, not food intoxication. Option D is incorrect as not all Bacillus species cause food intoxication.

Correct Answer: D

Rationale: The correct answer is D: Index of fecal coli-forms. Fecal coli-forms are bacteria found in the intestines of warm-blooded animals, including humans. Their presence in water indicates fecal contamination, which is a common source of hepatitis A transmission. Monitoring the index of fecal coli-forms can help identify potential sources of contamination and assess the risk of disease transmission. A: Number of coli-phages - Coli-phages are viruses that infect coliform bacteria and are not directly related to fecal contamination. B: Escherichia coli index - While E. coli is a type of coliform bacteria found in the intestines of warm-blooded animals, it alone may not be a reliable indicator of fecal contamination in water sources. C: Oxidability - Oxidability is a measure of the water's ability to react with oxygen and does not provide direct information about fecal contamination or the presence of pathogens like hepatitis A. In summary,

Correct Answer: B

Rationale: The correct answer is Staphylococcus saprophyticus (choice B). This bacterium is a common cause of urinary tract infections in young women. It typically grows on blood agar without hemolysis and appears as Gram-positive cocci under the microscope. Proteus mirabilis (choice A) is known for urease production, causing alkaline urine. Staphylococcus aureus (choice C) is a common cause of skin and soft tissue infections, not urinary tract infections. Pseudomonas aeruginosa (choice D) is an opportunistic pathogen often seen in nosocomial infections, not typically associated with urinary tract infections in healthy individuals.

Correct Answer: A

Rationale: The correct answer is A: Pseudomonas aeruginosa. Pseudomonas aeruginosa is known for producing a blue-green pigment called pyocyanin, which is characteristic of its colonies. This bacterium is commonly associated with purulent infections, especially in immunocompromised patients. Proteus vulgaris (B) is not typically associated with blue-green pigment production. Bacillus subtilis (C) is not a common pathogen in wound infections. Klebsiella pneumoniae (D) does not produce blue-green pigment and is more commonly associated with respiratory infections. Therefore, based on the clinical presentation and pigment production, Pseudomonas aeruginosa is the most likely causative agent in this case.

Correct Answer: B

Rationale: The correct answer is B: autoclaving. Autoclaving is a process that uses high pressure and steam to achieve temperatures above the boiling point of water, typically around 121-134°C. This high heat and pressure are effective in destroying spore forms of microorganisms, which are highly resistant structures that can withstand boiling temperatures. Autoclaving ensures the complete destruction of spores, making it an effective method for sterilization. Rationale: A: Pasteurization uses lower heat compared to autoclaving and is not sufficient to destroy spore forms. C: Freezing does not effectively destroy spore forms, as most microorganisms can survive freezing temperatures. D: Boiling, while effective for many microorganisms, may not be sufficient to destroy spore forms due to their high resistance, unlike autoclaving.