All of the following reactions are used for the serological identification of Yersinia pestis, EXCEPT:

Correct Answer: D

Rationale: The correct answer is D: Agglutination test type Gruber. This is because the Gruber agglutination test is not typically used for the serological identification of Yersinia pestis. The other options (A, B, C) are commonly used methods for serological identification of Y. pestis: A) Precipitation reaction involves the formation of a visible precipitate when Y. pestis antigen reacts with specific antibodies. B) Indirect hemagglutination test detects antibodies against Y. pestis by measuring the agglutination of red blood cells. C) Immunofluorescence test uses fluorescently labeled antibodies to detect specific antigens of Y. pestis. In summary, the Gruber agglutination test is not a standard method for serological identification of Yersinia pestis, unlike the other options provided.

Correct Answer: B

Rationale: The correct answer is B because the structure of the cytoplasmic membrane in gram-positive and gram-negative bacteria is fundamentally different. Gram-positive bacteria have a single-layered cytoplasmic membrane, while gram-negative bacteria have a double-layered cytoplasmic membrane with an additional outer membrane. This key difference is crucial for various functions such as antibiotic resistance and nutrient transport. Rationale for the incorrect choices: A: Gram-positive bacteria have a single-layered cytoplasmic membrane, not a double-layered one. C: Prokaryotes do have a cytoplasmic membrane, which is essential for maintaining cell integrity and regulating the passage of molecules. D: Gram-negative bacteria have a double-layered cytoplasmic membrane, not a three-layered one.

Correct Answer: A

Rationale: The correct answer is A: Entamoeba gingivalis. This organism matches the description provided in the question - unicellular with pseudopodia and ingested white blood cells, commonly found in dental plaque. Entamoeba gingivalis is known to inhabit the oral cavity and is associated with periodontal disease. Trichomonas vaginalis, Giardia lamblia, and Balantidium coli do not match the characteristics mentioned in the question and are not typically found in the oral cavity.

Correct Answer: D

Rationale: The correct answer is D because all three bacteria listed produce toxins that cause damage to host tissues. Clostridium tetani produces tetanospasmin toxin causing tetanus, Vibrio cholerae produces cholera toxin leading to severe diarrhea, and Staphylococcus aureus releases toxins causing tissue damage in various infections. Therefore, all of the above choices are correct in terms of producing toxins that harm host tissues.

Correct Answer: C

Rationale: The correct answer is C: Salmonella typhi. The patient's symptoms of diarrhea and the characteristics of non-lactose-fermenting, Gram-negative rods that are oxidase-positive and fermentative are consistent with Salmonella typhi. Salmonella typhi is the causative agent of typhoid fever, which presents with symptoms like diarrhea. Pseudomonas aeruginosa (choice A) is oxidase-positive but not fermentative. Escherichia coli (choice B) is both lactose-fermenting and oxidase-negative. Shigella dysenteriae (choice D) is oxidase-negative. Therefore, Salmonella typhi is the most likely causative agent based on the given information.

Correct Answer: D

Rationale: The correct answer is D: Agglutination test type Gruber. This is because the Gruber agglutination test is not typically used for the serological identification of Yersinia pestis. The other options (A, B, C) are commonly used methods for serological identification of Y. pestis: A) Precipitation reaction involves the formation of a visible precipitate when Y. pestis antigen reacts with specific antibodies. B) Indirect hemagglutination test detects antibodies against Y. pestis by measuring the agglutination of red blood cells. C) Immunofluorescence test uses fluorescently labeled antibodies to detect specific antigens of Y. pestis. In summary, the Gruber agglutination test is not a standard method for serological identification of Yersinia pestis, unlike the other options provided.

Correct Answer: D

Rationale: The correct answer is D: Fifth disease. Rubella virus is associated with Fifth disease, also known as erythema infectiosum. It is caused by parvovirus B19, not rubella virus. Mumps (choice A) is caused by the mumps virus. Roseola (choice B) is caused by human herpesvirus 6 or 7. Rubeola (choice C) is the scientific name for measles, caused by the measles virus. Therefore, the correct answer is Fifth disease as it is the disease associated with rubella virus.

Correct Answer: D

Rationale: The correct answer is D: To determine the children that need to receive BCG vaccination. The Mantoux test is used to identify individuals who have been exposed to Tuberculosis (TB) and need BCG vaccination for protection. Parotitis (choice A) is a viral infection unrelated to TB. Allergization rate toward rickettsia (choice B) and immune stress toward diphtheria (choice C) are not related to the Mantoux test or BCG vaccination. Therefore, choice D is the correct answer as it aligns with the purpose of the Mantoux test.

Correct Answer: B

Rationale: The correct answer is B: Blood agar. Pneumococci are fastidious bacteria that require enriched media for cultivation. Blood agar provides essential nutrients and growth factors needed for the growth of pneumococci. It contains sheep blood, which provides hemin and NAD, supporting the growth of these bacteria. Kligler iron agar (A) is used for differentiating enteric bacteria based on glucose and lactose fermentation. Apocholate citrate agar (C) is used for the selective isolation of Escherichia coli. Peptone water (D) is a non-selective medium used for the initial cultivation of microorganisms.