After its formation, the tetanus exotoxins:

Correct Answer: D

Rationale: Step-by-step rationale for why answer D is correct: 1. Tetanus exotoxins are produced by Clostridium tetani bacteria. 2. Once formed, the exotoxins can spread through the bloodstream. 3. Retrograde axonal transport allows the exotoxins to travel to the central nervous system (CNS). 4. In the CNS, the exotoxins can cause the symptoms of tetanus. 5. Options A, B, and C are incorrect as tetanus exotoxins do not follow those pathways. In summary, option D is correct as tetanus exotoxins can spread through the blood and reach the CNS via retrograde axonal transport, while the other options do not align with the known pathophysiology of tetanus.

Correct Answer: A

Rationale: The correct answer is A because spores are heat-resistant and require high temperatures for destruction. Autoclaving at 121°C for 15-20 minutes ensures spore destruction due to the high pressure and temperature. Choice B (heating to 80°C) is insufficient to destroy spores. Choice C (boiling at 100°C for 10 minutes) is also inadequate as spores require higher temperatures for elimination. Choice D (None of them) is incorrect as autoclaving is the standard method for spore sterilization.

Correct Answer: C

Rationale: The correct answer is C, Meat-peptone agar, as it provides a simple nutritive media for bacterial growth. This agar contains meat extract and peptone, which are good sources of nutrients for bacterial growth. Glucose broth (A) is a liquid medium with glucose but lacks other essential nutrients. Ascites (serum) agar (B) is a selective medium used for detecting pathogens in clinical samples. Blood agar (D) is a differential medium used to distinguish different types of bacteria based on hemolytic reactions. Meat-peptone agar is the simplest and most general-purpose medium among the options provided.

Correct Answer: B

Rationale: The correct answer is B because the capsule of bacteria is mainly composed of polysaccharides or polypeptides. This outer layer helps bacteria evade the host's immune system and protects them from desiccation. Peptidoglycan (choice A) is a component of the bacterial cell wall, not the capsule. Flagellin (choice C) is a protein component of the bacterial flagella, not the capsule. Choice D is incorrect as the capsule is indeed composed of polysaccharides or polypeptides.

Correct Answer: A

Rationale: The correct answer is A: Neisser. Bacterial capsules can be stained using the Neisser staining method because it specifically targets the capsule, making it visible under a microscope. Neisser staining involves using a combination of acidic and basic dyes to highlight the capsule structure. This method helps differentiate the capsule from the bacterial cell, aiding in the identification and study of capsules. Choices B, C, and D are incorrect because Cleat and Müller staining methods are not commonly used for staining bacterial capsules, and "None of the above" is incorrect as Neisser staining is a valid method for capsule staining.