A smear of sputum from the patient with suspected lobar pneumonia was stained with the use of the following stains and reagents: solution of gentian violet, Lugol’s solution, 960 alcohol, watermagenta. What staining method was applied in this case?

Correct Answer: A

Rationale: The correct answer is A: Gram staining method. The rationale is as follows: 1. Gentian violet is a primary stain used in the Gram staining procedure to stain the bacterial cells. 2. Lugol's solution is a mordant that helps to fix the crystal violet stain in Gram-positive bacteria. 3. 95% alcohol is used as a decolorizing agent in the Gram staining process to remove the stain from Gram-negative bacteria. 4. Watermagenta is not a standard reagent in the Gram staining method. Therefore, based on the reagents mentioned, the staining method applied in this case is the Gram staining method. Other choices are incorrect: B: Ziehl-Nielsen's - This staining method is used for acid-fast staining, not for lobar pneumonia. C: Romanovsky - This staining method is used for blood smears, not for bacterial staining in pneumonia. D: Neisser - This staining method is not commonly used in routine bacterial staining procedures.

Correct Answer: B

Rationale: The correct answer is B: Phage typing of the obtained culture. This method is used for typing Mycobacterium tuberculosis strains based on susceptibility to specific bacteriophages. If the culture shows susceptibility, it confirms the presence of M. tuberculosis. In this case, the absence of cord factor indicates that the bacteria are unlikely to be M. tuberculosis. Option A (Inoculation of laboratory animals) is unethical and not necessary when other methods are available. Option C (Allergy skin test) is not relevant for confirming a diagnosis of renal tuberculosis. Option D (Toxigenicity testing) is not specific for confirming or refuting a diagnosis of M. tuberculosis.

Correct Answer: A

Rationale: Rationale: 1. Thin, pale pink microorganisms with spiral coils suggest Treponema pallidum, the causative agent of syphilis. 2. Staining with Romanovsky-Giemsa method highlights the characteristic morphology of T. pallidum. 3. Syphilis presents with various stages and symptoms, correlating with the lymph node involvement. 4. Leptospirosis is caused by Leptospira interrogans, presenting with different clinical features. 5. Relapsing fever is caused by Borrelia species, which do not match the description. 6. Trypanosomiasis is caused by Trypanosoma species, which have distinct morphology and staining properties.

Correct Answer: D

Rationale: The correct answer is D: Loefler. This staining method is used for identifying Corynebacterium diphtheriae, the causative agent of diphtheria. It involves using methylene blue and basic fuchsin to stain the bacteria. The yellow bacilli with blue grains at their ends indicate the characteristic appearance of C. diphtheriae when stained using the Loefler method. Choice A: Ziehl-Nielsen is used for acid-fast bacteria like Mycobacterium tuberculosis. Choice B: Kozlovsky is not a standard staining method in microbiology. Choice C: Romanovsky is used for blood smears to identify malaria parasites.

Correct Answer: A

Rationale: The correct answer is A: Anaerobic. Pulpitis is an inflammatory condition of the dental pulp, often caused by bacterial infection. Anaerobic bacteria are commonly associated with dental infections, making it likely to find them in the inoculated culture medium. Anaerobic bacteria thrive in the absence of oxygen, which is typical of the dental environment. Acid-resistant (B) and acidophilic (C) microorganisms are not specifically linked to pulpitis. Haemolytic (D) microorganisms typically lyse red blood cells, which is not a characteristic feature of bacteria causing pulpitis. Therefore, the most likely microorganisms to be found in the culture medium would be anaerobic bacteria.

Correct Answer: C

Rationale: Step-by-step rationale for why C is correct: 1. Immunomodulators regulate the immune response. 2. Gamma-interferon is a cytokine that enhances immune function. 3. It helps fight infections and regulate inflammation. 4. Pentaxim and Hexacima are combination vaccines. 5. Rifampin is an antibiotic, not an immunomodulator. Summary: - A and B are combination vaccines. - D is an antibiotic. - C is the correct answer as gamma-interferon is an immunomodulator that regulates the immune response.

Correct Answer: A

Rationale: The correct answer is A: Toxoplasma. The crescent-shaped protozoa with pointed ends and blue cytoplasm described in the question are characteristic of Toxoplasma gondii, the causative agent of toxoplasmosis. Toxoplasma staining with Romanovsky-Giemsa method typically shows these specific morphological features. Plasmodium causes malaria and does not exhibit the described morphology. Leishmania and Trypanosoma are both kinetoplastid parasites, but they do not typically present as crescent-shaped protozoa with blue cytoplasm. Therefore, based on the specific characteristics described in the question, the correct answer is Toxoplasma.

Correct Answer: A

Rationale: The correct answer is A: Metronidazole. Parodontosis is a condition caused by bacterial infection, not protozoal infection. Metronidazole is an effective antibiotic commonly used to treat bacterial infections in the oral cavity, including those associated with parodontosis. Levamisole is an anthelmintic drug used to treat parasitic worm infections, not suitable for parodontosis. Griseofulvin is an antifungal medication used for fungal infections, not indicated for parodontosis. Mykoseptin is not a recognized drug and is likely a distractor. Therefore, based on the bacterial nature of parodontosis, Metronidazole is the appropriate choice.

Correct Answer: B

Rationale: The correct answer is B because lysozyme, lactoferrin, interferon, and complement are all humoral factors involved in natural resistance. Lysozyme and lactoferrin are antimicrobial proteins that help defend against pathogens. Interferon is a signaling protein that inhibits viral replication, while complement is a group of proteins that enhance the immune response by promoting inflammation and opsonization. Choices A, C, and D are incorrect: - Choice A mentions only macrophages, which are not humoral factors but rather phagocytic cells. - Choice C lists immune cells involved in adaptive immunity (B and T lymphocytes, NK cells) rather than humoral factors of natural resistance. - Choice D includes immunoglobulins, which are part of adaptive immunity, not natural resistance.