A smear of sputum from the patient with suspected lobar pneumonia was stained with the use of the following stains and reagents: solution of gentian violet, Lugol's solution, 96o alcohol, water-magenta. What staining method was applied in this case?

Correct Answer: A

Rationale: The correct answer is A: Gram staining method. This method involves staining with gentian violet, followed by Lugol's solution, 96% alcohol decolorization, and counterstaining with safranin or water-magenta. This staining sequence allows for the differentiation of bacteria based on their cell wall composition. In the case of lobar pneumonia, the causative agent is typically a bacterium with a cell wall that retains the crystal violet stain after decolorization with alcohol, indicating a Gram-positive bacterium. Summary of Incorrect Choices: B: Ziehl-Nielsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for lobar pneumonia. C: Romanovsky staining is primarily used in the identification of blood cells and blood parasites, not for bacteria. D: Neisser staining is not a commonly used staining method in microbiology for bacterial identification.

Correct Answer: D

Rationale: The correct answer is D: nonproprietary. The generic name of a drug is the official, nonproprietary name given to a drug by an organization such as the U.S. Food and Drug Administration (FDA). This name is not owned by any specific company and is used universally to identify the drug's active ingredient. The generic name helps to communicate the specific medication being prescribed and allows for cost-effective options for consumers. A: Trade name refers to the brand name given to a drug by a pharmaceutical company. B: Proprietary name is another term for the trade name, specific to the company that produces the drug. C: Chemical name refers to the exact chemical composition of the drug, which may not be user-friendly or easily recognized by healthcare professionals or consumers.

Correct Answer: C

Rationale: The correct answer is C (7.2-7.4) because most pathogenic bacteria and viruses thrive in a neutral pH environment close to 7.0. This pH range is optimal for their growth and survival. Choice A (5.0-5.5) is too acidic for most pathogens. Choice B (8.0-9.6) is too alkaline, which is not conducive for their survival. Choice D (4.2-4.4) is also too acidic and would likely inhibit the growth of pathogenic bacteria and viruses. Thus, a pH range of 7.2-7.4 provides the most suitable conditions for their proliferation.

Correct Answer: D

Rationale: The correct answer is D: Trichomonas tenax. Trichomonas tenax is a flagellated protozoan that is commonly found in the oral cavity, specifically in dental plaque. The description provided in the question matches the characteristics of Trichomonas tenax, such as unicellular structure, distinct layers in the cytoplasm, wide pseudopodia, and barely visible core. Entamoeba gingivalis (choice A) and Entamoeba histolytica (choice B) are amoebas, not flagellated protozoa, and they do not exhibit the characteristics described in the question. Lamblia (choice C) is also an incorrect choice as it is a different type of protozoan that does not match the description provided.

Correct Answer: C

Rationale: The correct answer is C: Endospore. Endospores are dormant, tough, and non-reproductive structures formed by certain bacteria as a response to unfavorable conditions. They help bacteria survive harsh environments such as extreme temperatures, desiccation, and exposure to harmful chemicals. The other choices, Flagella (A), Capsule (B), and Pili (D), do not directly contribute to bacterial survival in adverse conditions. Flagella aid in bacterial movement, capsules provide protection from immune system attacks, and pili are involved in attachment to surfaces or other cells. Endospores are specifically designed for survival during unfavorable conditions, making them the correct choice in this scenario.

Correct Answer: B

Rationale: The correct answer is B: Polymorphonuclear leukocyte. APCs are specialized immune cells that present antigens to T cells. B cells and dendritic cells are classic examples of APCs, as they can efficiently present antigens. Polymorphonuclear leukocytes, such as neutrophils, are not considered traditional APCs because they are primarily involved in phagocytosis and killing of pathogens rather than antigen presentation. Therefore, they do not play a significant role in initiating adaptive immune responses. The incorrect choices are A (B cell) and C (Dendritic cell) because they are both well-known antigen-presenting cells in the immune system. Choice D (All of the above) is incorrect because B cells and dendritic cells are indeed antigen-presenting cells.

Correct Answer: B

Rationale: The correct answer is B: IgM. IgM is the first antibody synthesized by the infant soon after birth. It is produced by B cells in response to initial exposure to antigens. IgA is mainly found in mucosal areas, IgG is transferred from mother to fetus, and IgD functions in the early stages of B cell development. Therefore, IgM is the most suitable choice as the antibody synthesized by the infant soon after birth.

Correct Answer: C

Rationale: Rationale: Schizogony is the process in protozoans where multiple nuclear divisions occur before cell division. This results in the formation of multiple daughter cells. - A: Budding is a form of asexual reproduction where a new organism grows from an outgrowth on the parent. - B: Mitosis is a type of cell division that results in two identical daughter cells. - D: Fragmentation is a form of asexual reproduction where an organism breaks into fragments, each developing into a new organism.

Correct Answer: A

Rationale: The correct answer is A: Shigella dysenteriae. Shigella causes dysentery, characterized by bloody diarrhea and abdominal pain. Shigella is a non-lactose-fermenting, Gram-negative rod. B: Salmonella typhi causes typhoid fever, not typically associated with abdominal pain and diarrhea. C: Escherichia coli, a common gut bacteria, can cause diarrhea but typically ferments lactose. D: Proteus vulgaris is not a common cause of gastrointestinal infection and is not typically associated with abdominal pain and diarrhea.