A patient with pneumonia had sputum stained by the Gram method. The smear revealed Gram-positive cocci in pairs with a capsule. What is the most likely causative agent?

Correct Answer: A

Rationale: The correct answer is A: Streptococcus pneumoniae. This bacterium is a common cause of pneumonia and is known to appear as Gram-positive cocci in pairs with a capsule. The presence of a capsule is a key characteristic of S. pneumoniae. Staphylococcus aureus (B) is a Gram-positive cocci but typically does not have a capsule. Neisseria meningitidis (C) is a Gram-negative diplococcus, not a Gram-positive cocci. Klebsiella pneumoniae (D) is a Gram-negative rod, not a Gram-positive cocci. Therefore, based on the staining pattern and the presence of a capsule, Streptococcus pneumoniae is the most likely causative agent in this case.

Correct Answer: D

Rationale: The correct answer is D: Gram-negative coccobacillus. Francisella tularensis is a Gram-negative coccobacillus because it has a unique shape that is between a coccus (spherical) and a bacillus (rod-shaped) and it stains pink/red in the Gram staining process. It is important to note that it is not a coccus (choice B) or a Gram-positive bacterium (choice A). Choice C is too general and does not specify the coccobacillus shape of F. tularensis. So, the correct answer is D due to its specific characteristics as a Gram-negative coccobacillus.

Correct Answer: A

Rationale: The correct answer is A: The lysozyme hydrolyzes the glycoside bonds between the N-acetylmuramic acid and the N-acetylglucosamine in the peptidoglycan of the cell wall. This is because lysozyme is an enzyme that specifically targets the peptidoglycan layer of bacterial cell walls, breaking down the structure by cleaving the glycosidic bonds between these two specific sugar molecules. This action weakens the cell wall, leading to bacterial cell lysis. The other choices are incorrect because lysozyme does not target phosphate bonds in phospholipids (B), disulfide bonds in immunoglobulins (C), or hydrogen bonds in bacterial capsules (D).

Correct Answer: C

Rationale: The correct answer is C: Weakly bound to the cell wall. The glycocalyx in bacteria is a protective layer that can be loosely attached to the cell wall or membrane. This loose attachment allows for flexibility and protection against desiccation and immune responses. Choice A is incorrect because the glycocalyx is not strongly bound to the cell wall, as it needs to be able to be shed easily for bacteria to escape host immune responses. Choice B is incorrect because the glycocalyx is not directly involved in bacterial movement, but rather serves as a protective barrier. Choice D is incorrect as the glycocalyx does play a role in bacterial physiology.

Correct Answer: A

Rationale: The correct answer is A: Bacterial ribosomes synthesize proteins. Ribosomes are cellular organelles responsible for protein synthesis by translating mRNA into amino acids. They are essential for the production of proteins needed for cell function. Choices B, C, and D are incorrect because ribosomes do not synthesize DNA, degrade toxic substances, or assist in cell division. DNA synthesis is carried out by DNA polymerase, degradation of toxic substances is typically done by enzymes, and cell division is primarily regulated by the cell cycle machinery.

Correct Answer: D

Rationale: The correct answer is D because the female reproductive system consists of the uterus, ovaries, vagina, and fallopian tubes. The ovaries produce eggs, the fallopian tubes transport the eggs to the uterus, where fertilization can occur. The vagina is the birth canal and where sperm is deposited during intercourse. The uterus is where a fertilized egg implants and a fetus develops. Choice A is incorrect because it includes the kidneys, which are part of the urinary system, not the reproductive system. Choice B is incorrect because it includes the bladder and duodenum, which are part of the urinary and digestive systems, respectively, not the reproductive system. Choice C is incorrect because it includes the bladder and ureter, which are part of the urinary system, not the reproductive system.

Correct Answer: C

Rationale: The correct answer is C because bacterial enzymes like amylase and protease catalyze biochemical reactions like digestion. Amylase breaks down carbohydrates, while protease breaks down proteins. This process is essential for bacterial metabolism and nutrient acquisition. Choice A (Break down toxins) is incorrect because bacterial enzymes are primarily involved in nutrient breakdown, not toxin degradation. Choice B (Facilitate movement) is incorrect as enzymes are not directly involved in bacterial movement. Choice D (Promote spore formation) is incorrect because enzymes like amylase and protease are not directly involved in spore formation.

Correct Answer: B

Rationale: The correct answer is B: Spirochete. Lyme disease is caused by the bacterium Borrelia burgdorferi, which is a spirochete. Spirochetes are spiral-shaped bacteria that move by rotating like a corkscrew, which is characteristic of Borrelia burgdorferi. A: Spirillum - Spirillum bacteria are helical-shaped but are not the causative agent for Lyme disease. C: Vibrio - Vibrio bacteria are curved or comma-shaped and are not associated with Lyme disease. D: Diplococcus - Diplococcus bacteria are spherical-shaped and typically occur in pairs, not the causative agent for Lyme disease. Therefore, the correct answer is B because Borrelia burgdorferi is a spirochete bacterium that causes Lyme disease.

Correct Answer: A

Rationale: The correct answer is A: Trichomonas vaginalis. Trichomonas vaginalis is a sexually transmitted protozoan that causes trichomoniasis. The pear-shaped flagellated protozoa with an undulating membrane described in the question match the morphology of Trichomonas vaginalis. It is typically found in vaginal discharge of infected individuals. Giardia lamblia (B) is a different protozoan that causes giardiasis and has a distinct morphology of a teardrop shape with flagella. Trypanosoma brucei (C) causes African sleeping sickness and Leishmania donovani (D) causes visceral leishmaniasis, both of which have different morphologies and clinical presentations compared to Trichomonas vaginalis.