Correct Answer: A

Rationale: The correct answer is A: Streptococcus pneumoniae. This bacterium is a Gram-positive cocci that often appear in pairs and can have a capsule. It is a common cause of sore throat and fever, known as streptococcal pharyngitis. Staphylococcus aureus (B) is a Gram-positive cocci but does not typically form pairs. Neisseria meningitidis (C) is a Gram-negative diplococcus and is more associated with meningitis. Klebsiella pneumoniae (D) is a Gram-negative rod and not typically associated with sore throat and fever.

Correct Answer: A

Rationale: The correct answer is A: Gram staining method. This method involves staining with gentian violet, followed by Lugol's solution, 96% alcohol decolorization, and counterstaining with safranin or water-magenta. This staining sequence allows for the differentiation of bacteria based on their cell wall composition. In the case of lobar pneumonia, the causative agent is typically a bacterium with a cell wall that retains the crystal violet stain after decolorization with alcohol, indicating a Gram-positive bacterium. Summary of Incorrect Choices: B: Ziehl-Nielsen staining is used for acid-fast bacteria like Mycobacterium tuberculosis, not for lobar pneumonia. C: Romanovsky staining is primarily used in the identification of blood cells and blood parasites, not for bacteria. D: Neisser staining is not a commonly used staining method in microbiology for bacterial identification.

Correct Answer: A

Rationale: The correct answer is A: Aspergillosis and candidiasis. Galactomannan is a polysaccharide found in Aspergillus cell walls and is detected in patients with invasive aspergillosis. Free mannan is a polysaccharide found in Candida cell walls and is detected in patients with candidiasis. Therefore, detection of galactomannan and free mannan is specific for diagnosing Aspergillosis and candidiasis. Choices B, C, and D are incorrect as HIV infection, syphilis, and leptospirosis do not involve the detection of galactomannan or free mannan for diagnosis.

Correct Answer: B

Rationale: The correct answer is B because endotoxins are part of the outer membrane of gram-negative bacteria. When these bacteria undergo cell lysis, the endotoxins are released, causing harmful effects. This process does not occur during bacterial replication (choice A), antibody production (choice C), or formation of spores (choice D). Bacterial replication involves the creation of new bacterial cells, not the release of endotoxins. Antibody production is the immune response to pathogens, not the direct cause of endotoxin release. Spores are dormant structures formed by some bacteria, which are not directly related to the release of endotoxins.

Correct Answer: D

Rationale: The correct answer is D: Q-fever. Q-fever is typically an infection that affects the lower respiratory tract, causing symptoms like pneumonia. Haemophilus influenzae pharyngitis (choice A) is an upper respiratory tract infection. Ozeana (choice B) and diphtherial croup (choice C) are not specific infections related to the lower respiratory tract. Q-fever stands out as the correct choice due to its association with lower respiratory tract infections.