A patient underwent esophagogastroduodenoscopy. Analysis of the biopsy material enabled doctors to diagnose him with helicobacteriosis. What property of the bacteria found in this patient had to be obligatory taken into account during their cultivation?

Correct Answer: A

Rationale: Step-by-step rationale for why choice A is correct: 1. Helicobacter pylori, the bacteria causing helicobacteriosis, is microaerophilic, requiring low oxygen levels. 2. Cultivation conditions must mimic the natural environment of the bacteria. 3. Failure to provide a microaerophilic environment can hinder bacterial growth. 4. Thus, the ability to cultivate these bacteria requires consideration of their microaerophilic nature. Summary: - Choice A is correct because Helicobacter pylori is a microaerophilic bacteria. - Choice B (Presence of urease) is incorrect as it is a characteristic used for diagnosis, not cultivation. - Choice C (Absence of spores and capsules) is incorrect as it does not relate to cultivation requirements. - Choice D (Colonization of gastral cells) is incorrect as it pertains to bacterial behavior, not cultivation needs.

Correct Answer: C

Rationale: The correct answer is C because chills, fever, and loss of consciousness are not typical symptoms of botulism. Botulism is characterized by descending, flaccid paralysis (choice A), dry mouth, difficulty swallowing, and speaking (choice B), and blurred vision, diplopia (choice D). These symptoms result from the neurotoxin produced by Clostridium botulinum, leading to muscle weakness and paralysis. Chills, fever, and loss of consciousness are more commonly associated with infections or other conditions, not botulism.

Correct Answer: B

Rationale: The correct answer is B: mycorrhizae. Mycorrhizae are symbiotic fungi that form a mutualistic relationship with plant roots, aiding in the absorption of minerals and water. They enhance the plant's nutrient uptake capacity, promoting plant growth and health. Decomposers (A) break down dead organic matter, yeasts (C) are single-celled fungi used in fermentation, and molds (D) are fungi that grow in multicellular filaments. These options do not specifically assist plant roots in mineral and water absorption like mycorrhizae do.

Correct Answer: A

Rationale: The correct answer is A: Escherichia coli. E. coli is a Gram-negative rod that ferments lactose. It is a common cause of gastroenteritis presenting with fever and diarrhea. Salmonella typhi does not ferment lactose and causes typhoid fever. Shigella dysenteriae causes dysentery and does not ferment lactose. Proteus vulgaris does not typically cause gastroenteritis and is not known for lactose fermentation.

Correct Answer: A

Rationale: Correct Answer: A Rationale: 1. Enteropathogenic E. coli (EPEC) attach to enterocytes via a type III secretion system. 2. Once attached, they form attaching and effacing lesions on the microvilli, leading to microvillus destruction. 3. This mechanism allows EPEC to cause diarrhea by disrupting the normal absorptive function of the intestines. Summary: - B (They are invasive and produce enterotoxin) is incorrect because EPEC are non-invasive and do not produce enterotoxins. - C (Cause disease clinically similar to bacterial) is vague and does not provide specific information about EPEC pathogenesis. - D (None of the above) is incorrect as A is the correct explanation for EPEC pathogenicity.

Correct Answer: A

Rationale: The correct answer is A: Escherichia coli. E. coli is commonly associated with UTIs due to its ability to colonize the urinary tract. It possesses adhesins that allow it to attach to the bladder wall, causing infection. E. coli is also a common inhabitant of the intestinal tract, making it easily able to migrate to the urinary system. Explanation of incorrect choices: B: Clostridium botulinum - This bacterium causes botulism, a rare foodborne illness, not UTIs. C: Staphylococcus aureus - Often associated with skin infections and abscesses, not UTIs. D: Bacillus cereus - Known for causing food poisoning, not UTIs.

Correct Answer: A

Rationale: The correct answer is A: Ziehl-Neelsen staining method. This method is used to detect acid-fast bacteria like Mycobacterium tuberculosis. Step-by-step rationale: 1. Acid-fast bacteria resist decolorization with acid-alcohol. 2. The red bacilli seen in the smear indicate acid-fast bacteria. 3. Ziehl-Neelsen stain uses carbol fuchsin to stain acid-fast bacteria red. 4. The blue background is likely the counterstain methylene blue. Incorrect choices: B: Gram stain is for categorizing bacteria into Gram-positive or Gram-negative, not specific for acid-fast bacteria. C: Giemsa stain is used for blood films and detecting parasites, not acid-fast bacteria. D: Neisser stain is not a standard microbiological staining method.

Correct Answer: D

Rationale: The correct answer is D: it is a live attenuated vaccine. This is true because the mumps vaccine is indeed a live attenuated vaccine, meaning it contains a weakened form of the virus to stimulate an immune response without causing the disease. This type of vaccine provides long-lasting immunity. A, B, and C are incorrect: A: the vaccine is recommended, not obligatory - this is incorrect because the mumps vaccine is typically recommended for all individuals to prevent the spread of the disease. B: the vaccine is not given in combination with other vaccines - this is incorrect as the mumps vaccine is often given in combination with measles and rubella vaccines as the MMR vaccine. C: the vaccine is administered orally - this is incorrect because the mumps vaccine is usually administered via injection, not orally.

Correct Answer: D

Rationale: The correct answer is D: Transcription. This is the process where the DNA sequence is copied into an RNA molecule. It is the initial step in gene expression. A: RNA splicing is the process of removing introns from pre-mRNA to create a mature mRNA molecule. B: Translation is the process of synthesizing proteins using the information from mRNA. C: Transposition is the movement of genetic elements within a genome. In summary, transcription specifically refers to the synthesis of RNA from DNA, making it the correct choice compared to the other options.