A patient has a suspected pneumonia. In his sputum there were revealed gram-positive diplococci, prolonged with the slightly pointed opposite ends. What microorganisms are revealed in the sputum?

Correct Answer: A

Rationale: Rationale: The description matches that of Streptococcus pneumoniae, which appears as gram-positive diplococci with slightly pointed opposite ends. Staphylococcus aureus, Neisseria gonorrhoeae, and Neisseria meningitidis do not match the specific characteristics described in the question. Staphylococcus aureus appears in grape-like clusters, while Neisseria species are typically seen as diplococci but lack the pointed shape described. Therefore, the correct answer is A: Staphylococcus aureus.

Correct Answer: D

Rationale: The correct answer is D, All of the above. Clostridium botulinum produces botulinum toxin causing botulism, Vibrio cholerae produces cholera toxin leading to severe diarrhea, and certain strains of Escherichia coli produce toxins causing gastrointestinal illness. Each bacterium has specific exotoxins that can result in severe gastrointestinal symptoms. Therefore, all three choices are correct in producing exotoxins that can cause severe gastrointestinal illness.

Correct Answer: A

Rationale: The correct answer is A: Vibrio cholerae. This bacterium produces the cholera toxin, an enterotoxin that causes profuse watery diarrhea by disrupting the normal function of the intestines. Vibrio cholerae is specifically known for causing cholera, a severe diarrheal disease. Clostridium botulinum (B) produces botulinum toxin, causing botulism (paralysis, not diarrhea). Escherichia coli (C) can produce enterotoxins, but the question specifies a specific enterotoxin causing diarrhea, which is not a characteristic of all strains of E. coli. Streptococcus pneumoniae (D) is a common cause of respiratory infections but does not produce an enterotoxin causing diarrhea.

Correct Answer: C

Rationale: The correct answer is C: (+RNA). This indicates that the scientist found a positive-sense RNA strand that can be directly translated by host cell machinery to produce viral proteins. Positive-sense RNA can act as messenger RNA, allowing immediate protein synthesis during viral replication. Choice A (RNA major) and D (RNA minor) are not standard terms used to describe RNA strands in virology. Choice B (-RNA) indicates a negative-sense RNA strand, which requires transcription to form a complementary positive-sense strand before protein synthesis. Thus, the identification of a strand of RNA that directly codes for viral proteins aligns with the characteristics of a positive-sense RNA strand.

Correct Answer: C

Rationale: The correct answer is C: Kirby-Bauer disk diffusion test. This test is used to determine the antibiotic susceptibility of bacteria by measuring the zone of inhibition around antibiotic-soaked paper disks. It helps identify which antibiotics are effective against the bacteria being tested. This test is crucial in guiding antibiotic treatment decisions. A: Gram stain is used to classify bacteria based on their cell wall composition and morphology, not for antibiotic susceptibility testing. B: Catalase test is used to differentiate between catalase-positive and catalase-negative bacteria, not for antibiotic susceptibility testing. D: Endospore stain is used to detect the presence of endospores in bacteria, not for antibiotic susceptibility testing.

Correct Answer: A

Rationale: The presence of rods with polar dark blue granules stained by Neisser's method indicates metachromatic granules consistent with Corynebacterium diphtheriae. The characteristic staining pattern is due to the presence of polar granules in the bacterial cells, a feature specific to Corynebacterium diphtheriae. Bacillus anthracis, Clostridium tetani, and Escherichia coli do not exhibit this staining pattern or morphology typical of Corynebacterium diphtheriae, making them incorrect choices.

Correct Answer: B

Rationale: The correct answer is B: Rhodophyta. Rhodophyta is the phylum of red algae, which are known to contain agar in their cell walls. Agar is a gelatinous substance derived from these algae. Chrysophyta (A), Chlorophyta (C), and Phaeophyta (D) do not typically contain agar in their cell walls. Chrysophyta includes diatoms, Chlorophyta includes green algae, and Phaeophyta includes brown algae, none of which are known for agar production. Therefore, Rhodophyta is the correct choice based on the presence of agar in their cell walls.

Correct Answer: D

Rationale: The correct answer is D because all three bacteria (Clostridium botulinum, Salmonella enterica, Escherich:ia coli) are known to produce toxins causing foodborne illnesses. Clostridium botulinum produces botulinum toxin, causing botulism. Salmonella enterica produces toxins leading to salmonellosis. Escherichia coli can produce toxins like Shiga toxin causing food poisoning. Choices A, B, and C are incorrect because each bacterium is individually known to produce toxins causing foodborne illnesses, thus the correct answer is D.

Correct Answer: A

Rationale: The correct answer is A: Tryptic soy agar is a general-purpose medium suitable for cultivating a wide range of microorganisms. It provides essential nutrients for growth without inhibiting specific types of bacteria. Blood agar (B), Mannitol salt agar (C), and MacConkey agar (D) are selective or differential media designed to promote the growth of specific types of bacteria or differentiate between different bacterial species. Therefore, they are not considered general-purpose mediums.