A patient has a necrotizing phlegmon of his lower extremity. A doctor suspects a gas gangrene. Microscopy reveals grampositive bacilli. In order to confirm the diagnosis further bacteriological tests should include inoculation of the material into the following nutrient medium:

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Mastering Microbiology Test Bank Questions

Question 1 of 9

A patient has a necrotizing phlegmon of his lower extremity. A doctor suspects a gas gangrene. Microscopy reveals grampositive bacilli. In order to confirm the diagnosis further bacteriological tests should include inoculation of the material into the following nutrient medium:

Correct Answer: A

Rationale: The correct answer is A: Kitt-Tarozzi medium. This medium is specifically designed to culture Clostridium species, the causative agents of gas gangrene. Clostridium species are gram-positive bacilli that produce gas during their metabolism. Inoculating the material into Kitt-Tarozzi medium allows for the selective growth of Clostridium species, aiding in the confirmation of a gas gangrene diagnosis. Other choices are incorrect: B: Endo agar is used for the isolation of gram-negative enteric bacteria. C: Meat-peptone agar is a general-purpose medium and not specific for Clostridium species. D: Levine agar is used for the isolation of Salmonella and Shigella species, not Clostridium.

Question 2 of 9

Treponemae are motile because of:

Correct Answer: A

Rationale: The correct answer is A: axial filaments. Treponemae are motile due to their unique structure of axial filaments, also known as endoflagella. These axial filaments are located within the periplasmic space of the organism and are responsible for the corkscrew-like motility of Treponemae. The other choices (B: mans peritrichous filaments, C: single monotrichous filament, D: fimbriae) do not play a role in the motility of Treponemae. Peritrichous filaments and single monotrichous filaments are not present in Treponemae, and fimbriae are primarily involved in adhesion rather than motility.

Question 3 of 9

Flagellin is:

Correct Answer: A

Rationale: Flagellin is a protein component of bacterial flagella. It is not a carbohydrate, staining method, or an organelle. The correct answer is A because flagellin is a structural protein that forms the flagellar filament. It helps in bacterial motility and pathogenicity. Therefore, the correct choice is A.

Question 4 of 9

Chlorine belongs to which of the following chemical groups?

Correct Answer: A

Rationale: The correct answer is A: Halogens. Chlorine belongs to the halogens group because it is a nonmetal element in group 17 of the periodic table. Halogens are highly reactive elements that have 7 electrons in their outer shell, making them eager to gain an electron to achieve stability. Chlorine specifically is a diatomic molecule with the chemical symbol Cl and atomic number 17. Option B: Heavy metals are elements with high atomic weights and densities, such as lead and mercury, which are not related to chlorine. Option C: Phenols are a class of organic compounds containing a hydroxyl group bonded to an aromatic ring, not related to chlorine. Option D: Alcohols are organic compounds characterized by the presence of a hydroxyl group (-OH), also not related to chlorine.

Question 5 of 9

A 34-year-old male visited Tajikistan. After return, he complains of fever up to 40oC which occurs every second day and is accompanied by chills, sweating. Hepatosplenomegaly is present. Blood test results: RBC- 3x1012/l, b- 80 g/l, WBC- 4x109/l, eosinophils - 1%, stab neutrophils - 5%, segmented neutrophils - 60%, lymphocytes - 24%, monocytes - 10%, ESR - 25 mm/h. What is the provisional diagnosis?

Correct Answer: A

Rationale: The correct answer is A: Malaria. The patient's travel history to an endemic area like Tajikistan, along with symptoms of fever with chills, sweating, and hepatosplenomegaly, are classic for malaria. The blood test results show anemia (low RBC and hemoglobin levels), which can occur in malaria due to hemolysis. The presence of eosinophils and lymphocytes are not typical for malaria, but the overall clinical picture fits. Infectious mononucleosis (choice B) typically presents with sore throat, lymphadenopathy, and atypical lymphocytosis, which are not seen in this case. Sepsis (choice C) is characterized by systemic inflammatory response syndrome and organ dysfunction due to infection, which does not align with the symptoms and findings in the case. Typhoid fever (choice D) usually presents with gradual onset of sustained fever, abdominal pain, headache, and constipation, which are not present in this patient

Question 6 of 9

Factors for transmission of nosocomial infections are:

Correct Answer: D

Rationale: The correct answer is D because all the factors listed can contribute to the transmission of nosocomial infections. Medical dressings can harbor pathogens, breast milk can be a source of infection if not handled properly, and toys can serve as fomites for spreading germs. Choosing any other option would be incorrect as they all play a role in facilitating the transmission of nosocomial infections. It is important to consider all potential sources of infection in healthcare settings to effectively prevent the spread of nosocomial infections.

Question 7 of 9

Precipitation reaction can be converted into agglutination reaction by coating soluble antigen onto

Correct Answer: D

Rationale: The correct answer is D: All of these. Coating soluble antigen onto Bentonite particles, RBCs, or Latex particles can convert a precipitation reaction into an agglutination reaction. This is because soluble antigens bind to their specific antibodies, causing the particles to clump together, visible as agglutination. Bentonite particles, RBCs, and Latex particles all provide surfaces for the antigen-antibody interaction to occur, leading to agglutination. Therefore, all of these options are correct for converting precipitation reactions to agglutination reactions.

Question 8 of 9

Algae that contain agar in their cell walls belong to:

Correct Answer: B

Rationale: The correct answer is B: Rhodophyta. Rhodophyta is the phylum of red algae, which are known to contain agar in their cell walls. Agar is a gelatinous substance derived from these algae. Chrysophyta (A), Chlorophyta (C), and Phaeophyta (D) do not typically contain agar in their cell walls. Chrysophyta includes diatoms, Chlorophyta includes green algae, and Phaeophyta includes brown algae, none of which are known for agar production. Therefore, Rhodophyta is the correct choice based on the presence of agar in their cell walls.

Question 9 of 9

A female who had been continuously taking antibiotics for an intestinal infection developed a complication manifested by inflammation of the oral mucosa and white deposit. Bacteriological study of the deposit samples revealed yeast fungi Candida albicans. Which of the following medications is indicated for the treatment of this complication?

Correct Answer: A

Rationale: Rationale for Correct Answer (A: Fluconazole): Fluconazole is an antifungal medication used to treat yeast infections caused by Candida albicans. It targets the fungal cell membrane, disrupting its structure and function. This makes it an effective treatment for oral thrush, which is caused by Candida overgrowth in the oral mucosa. The continuous use of antibiotics can disrupt the normal balance of microorganisms in the body, leading to fungal overgrowth. Fluconazole specifically targets the yeast fungi without affecting bacteria, making it the appropriate choice for this complication. Summary of Incorrect Choices: B: Biseptol - Biseptol is an antibiotic that targets bacteria, not fungi like Candida albicans. C: Tetracycline - Tetracycline is also an antibiotic that targets bacteria, not fungi. D: Furazolidone - Furazolidone is an antibiotic primarily used for treating bacterial infections, not fungal infections like oral thrush caused by Candida

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