A microscope that provides a three-dimensional image of a specimen is a

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microbiology chapter 10 test bank Questions

Question 1 of 9

A microscope that provides a three-dimensional image of a specimen is a

Correct Answer: D

Rationale: The correct answer is D: scanning electron microscope. A scanning electron microscope provides a three-dimensional image of a specimen by scanning a focused electron beam across the surface of the specimen, detecting the electrons that are emitted from the surface, and creating a detailed image. This is achieved through the detection of secondary electrons, backscattered electrons, and X-rays emitted from the specimen surface. A dark-field microscope (choice A) utilizes oblique lighting to enhance contrast and is not specifically designed to provide a 3D image. A transmission electron microscope (choice B) uses a beam of electrons transmitted through a specimen to create a 2D image at high magnification. A bright-field microscope (choice C) is a basic microscope that illuminates a specimen with uniform light and does not provide a 3D image like a scanning electron microscope does.

Question 2 of 9

Method for rapid diagnosis of some viral infections is

Correct Answer: D

Rationale: The correct answer is D because utilizing all three methods—immunofluorescence, viral neutralization reaction, and electron microscopy—provides a comprehensive and rapid diagnosis of various viral infections. Immunofluorescence detects viral antigens directly, viral neutralization reaction demonstrates the presence of specific antibodies, and electron microscopy visualizes viral particles. This combination ensures accurate and quick identification of different viruses. Choices A, B, and C alone may not cover all aspects of viral diagnosis, making them less effective compared to the comprehensive approach of using all three methods.

Question 3 of 9

Which of the following bacteria can form spores that are resistant to extreme conditions?

Correct Answer: B

Rationale: The correct answer is B: Bacillus subtilis. Bacillus subtilis is a Gram-positive bacterium known for forming endospores, which are resistant structures that allow the bacteria to survive harsh conditions like heat, radiation, and desiccation. This ability to form spores helps Bacillus subtilis to endure unfavorable environments. Staphylococcus aureus (A), Pseudomonas aeruginosa (C), and Neisseria gonorrhoeae (D) do not form spores and lack the same level of resistance to extreme conditions as Bacillus subtilis.

Question 4 of 9

A patient with a painful ulcer had a wound culture revealing Gram-negative rods with a characteristic 'safety pin' appearance. What is the most likely causative agent?

Correct Answer: A

Rationale: The correct answer is A: Yersinia pestis. Y. pestis is a Gram-negative rod that exhibits a 'safety pin' appearance on staining due to bipolar staining. This bacterium is the causative agent of plague, which can present as painful ulcers known as buboes. Y. pestis is transmitted by fleas and can cause outbreaks in humans. Summary: - B: Francisella tularensis causes tularemia, not typically associated with painful ulcers. - C: Brucella abortus causes brucellosis, which does not typically present with painful ulcers. - D: Clostridium botulinum causes botulism, characterized by muscle weakness and paralysis, not painful ulcers.

Question 5 of 9

A patient with diarrhea had a stool sample that revealed Gram-negative rods that fermented lactose. What is the most likely causative agent?

Correct Answer: A

Rationale: The correct answer is A: Escherichia coli. Step-by-step rationale: 1. Gram-negative rods: E. coli is a Gram-negative rod bacterium. 2. Fermented lactose: E. coli is known to ferment lactose. 3. Common cause of diarrhea: E. coli is a common causative agent of infectious diarrhea. Summary: B: Salmonella typhi causes typhoid fever, not typically associated with lactose fermentation. C: Shigella dysenteriae causes dysentery, not known for lactose fermentation. D: Proteus vulgaris is not a common cause of infectious diarrhea and does not typically ferment lactose.

Question 6 of 9

Not many bacterial species in nature form spores. Which of the following genera have this ability?

Correct Answer: C

Rationale: The correct answer is C: Bacillus, Clostridium. Both Bacillus and Clostridium are known to form spores. Bacillus species produce endospores, which are resistant structures for survival in harsh conditions, while Clostridium species also form spores for protection and dispersal. The other choices are incorrect because Borrelia and Treponema are spirochetes and do not form spores, Bacteroides and Fusobacterium are anaerobic bacteria that do not produce spores, and Bordetella and Brucella are non-spore forming bacteria as well.

Question 7 of 9

A patient has severe catarrhal symptoms. Material growth on Bordet-Gengou agar showed mercury- drop like colonies. Examination of the blood smears revealed some small ovoid gram-negative bacilli sized 1-3 microns. What microorganisms were isolated?

Correct Answer: A

Rationale: The correct answer is A: Bordetella. The presence of mercury-drop like colonies on Bordet-Gengou agar indicates Bordetella pertussis. The small ovoid gram-negative bacilli sized 1-3 microns are characteristic of Bordetella species. Catarrhal symptoms are typical of pertussis caused by Bordetella pertussis. Summary of other choices: B: Corynebacteria do not typically exhibit mercury-drop like colonies or small ovoid shapes. C: Mycobacteria are acid-fast bacilli and do not grow on Bordet-Gengou agar. D: Meningococcus is a gram-negative diplococcus and does not match the description provided.

Question 8 of 9

Measures that can be taken to prevent bacterial vaginitis include:

Correct Answer: D

Rationale: The correct answer is D because bacterial vaginitis is often caused by an imbalance in the vaginal flora, which can be disrupted by frequent douching (eliminating beneficial bacteria), tight clothing (restricting airflow), and non-breathable underwear. Avoiding tight pants and using cotton underwear allow for better ventilation, promoting a healthy vaginal environment. Infrequent douching helps maintain the natural balance of bacteria in the vagina. Choices A and B are incorrect because they recommend practices that can disrupt the vaginal flora and increase the risk of bacterial vaginitis. Choice C is partially correct but lacks the emphasis on avoiding frequent douching, which is crucial in preventing bacterial vaginitis.

Question 9 of 9

Which is not part of the normal skin microflora

Correct Answer: B

Rationale: The correct answer is B: serratia spp. This is because serratia spp. are not typically found in the normal skin microflora. Normal skin microflora primarily consist of bacteria such as diphtheroids, alpha-hemolytic streptococci, and epidermidis. Serratia spp. are more commonly associated with healthcare-associated infections rather than being a part of the skin's natural bacterial flora. Therefore, choice B is the correct answer. Choices A, C, and D are incorrect as they are commonly found in the normal skin microflora.

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