Correct Answer: A

Rationale: Step-by-step rationale for why the correct answer is A (Cholera): 1. Gram-negative curved mobile monotrichous bacilli: Vibrio cholerae, the causative agent of cholera, fits this description. 2. Do not produce spores or capsules: Vibrio cholerae does not produce spores or capsules. 3. Require alkaline reaction (pH 8.5-9.5): Vibrio cholerae thrives in alkaline environments. 4. Enteric infection: Cholera is a classic example of an enteric infection transmitted through contaminated food or water. Summary of why other choices are incorrect: B: Shigellosis is caused by Shigella species, which are gram-negative, but they are not curved bacilli and do not require an alkaline pH. C: Typhoid fever is caused by Salmonella Typhi, which are gram-negative but are not curved bacilli and do not require an alkaline pH.

Correct Answer: A

Rationale: The correct answer is A: Tuberculine. The Mantoux test involves injecting tuberculin, a protein derivative of Mycobacterium tuberculosis, under the skin. This test helps determine if a person has been exposed to the bacteria that cause TB. Administering tuberculin is crucial for the Mantoux test as it triggers a delayed-type hypersensitivity reaction in individuals previously exposed to TB. Choice B (BCG vaccine) is incorrect because BCG does not interfere with the Mantoux test results. Choice C (DPT vaccine) is incorrect as it is used for immunization against diphtheria, pertussis, and tetanus, not for diagnosing TB. Choice D (Tularin test) is incorrect as it is not a recognized immunobiological agent for TB diagnosis.

Correct Answer: D

Rationale: The correct answer is D: Cytoplasmic membrane - essential. The cytoplasmic membrane is a crucial structure in bacteria as it regulates the passage of substances in and out of the cell, provides structural support, and is essential for maintaining cell integrity. Without a functional cytoplasmic membrane, bacteria would not be able to survive. A: Nucleus is incorrect because bacteria do not have a true nucleus. B: Capsule - non-essential is incorrect because while the capsule provides protection and helps in adherence, it is not essential for bacterial survival. C: Mitochondria is incorrect because bacteria do not have mitochondria; they generate energy through other mechanisms. In summary, the cytoplasmic membrane is essential for bacterial survival, making it the correct choice among the options provided.

Correct Answer: B

Rationale: The correct answer is B because the main target of the toxin produced by Vibrio cholerae is the epithelial cells of the small intestine. This toxin, known as cholera toxin, binds to the surface of these cells and disrupts their normal function, leading to the characteristic watery diarrhea seen in cholera. The other choices are incorrect because macrophages are not the main target, RBCs are not directly affected by the toxin, and the stomach epithelial cells are not specifically targeted by Vibrio cholerae toxin.

Correct Answer: A

Rationale: The correct answer is A: Toxoplasma. Toxoplasma gondii is a protozoan parasite that can infect the brain and cause vision loss. The description of half-moon-shaped organisms with a pointed end matches the appearance of Toxoplasma under the microscope. Toxoplasma is known to cause a condition called toxoplasmosis, which can lead to neurological symptoms such as vision loss. Leishmania, Lamblia, Amoeba, and Trichomonad are not known to cause brain infections or vision loss, making them incorrect choices.