A Gram-stained smear from a wound infection revealed Gram-positive cocci arranged in clusters. The bacteria were catalase-positive and coagulase-negative. What is the most likely causative agent?

Correct Answer: A

Rationale: The correct answer is A: Staphylococcus epidermidis. The presence of Gram-positive cocci arranged in clusters, catalase-positive, and coagulase-negative characteristics are indicative of Staphylococcus species. Staphylococcus epidermidis is a common skin commensal that causes wound infections. Staphylococcus aureus is coagulase-positive and often causes more aggressive infections. Streptococcus pyogenes is catalase-negative and typically forms chains, not clusters. Enterococcus faecalis is catalase-negative and commonly found in the GI tract, not a common cause of wound infections.

Correct Answer: A

Rationale: The correct answer is A: Staphylococcus epidermidis. Step 1: Gram-positive cocci in clusters suggest staphylococci. Step 2: Catalase-positive and coagulase-negative narrows it down to Staphylococcus epidermidis, as it is catalase-positive and coagulase-negative. Staphylococcus aureus (B) is catalase-positive and coagulase-positive. Streptococcus pyogenes (C) is catalase-negative. Enterococcus faecalis (D) is catalase-negative and coagulase-negative.

Correct Answer: A

Rationale: The correct answer is A: Escherichia coli. E. coli is a Gram-negative rod that typically has a greenish hue on MacConkey agar due to its ability to ferment lactose, producing acid that changes the pH indicator to green. E. coli is a common cause of diarrhea, particularly in cases of traveler's diarrhea. Salmonella (B), Shigella (C), and Klebsiella (D) are also Gram-negative rods but do not typically show a greenish hue on MacConkey agar and are less commonly associated with causing diarrhea compared to E. coli.

Correct Answer: B

Rationale: The correct answer is B: Bacillus subtilis. Bacillus subtilis is known for its ability to resist heat and radiation due to its formation of endospores, which are highly resistant structures that protect the bacterium from harsh environmental conditions. This resilience allows Bacillus subtilis to survive in extreme environments where other bacteria cannot. Explanation of other choices: A: Escherichia coli is not known for its exceptional resistance to heat and radiation. C: Pseudomonas aeruginosa is not particularly resistant to heat and radiation compared to Bacillus subtilis. D: Clostridium perfringens is not as well-known for its heat and radiation resistance as Bacillus subtilis.

Correct Answer: A

Rationale: The correct answer is A: Streptococcus pyogenes. Rationale: 1. Gram-positive cocci in chains: S. pyogenes is a Gram-positive cocci in chains. 2. Catalase-negative: S. pyogenes is catalase-negative. 3. Beta-hemolytic: S. pyogenes exhibits beta-hemolysis. Summary of other choices: B: Streptococcus pneumoniae is alpha-hemolytic and optochin-sensitive. C: Enterococcus faecalis is catalase-negative, but not typically beta-hemolytic. D: Staphylococcus aureus is catalase-positive and coagulase-positive.