A child with foul-smelling diarrhea had trophozoites with two nuclei and flagella identified in stool microscopy. What is the causative agent?

Correct Answer: A

Rationale: The correct answer is A: Giardia lamblia. Giardia lamblia is a protozoan parasite that causes foul-smelling diarrhea with trophozoites having two nuclei and flagella in stool microscopy. The presence of flagella and two nuclei is characteristic of Giardia lamblia. Entamoeba histolytica (Choice B) causes amoebic dysentery with trophozoites containing ingested RBCs. Trichomonas vaginalis (Choice C) is a sexually transmitted protozoan causing vaginitis with a distinctive pear-shaped trophozoite. Balantidium coli (Choice D) is a ciliated protozoan causing diarrhea with trophozoites containing a single nucleus.

Correct Answer: A

Rationale: The correct answer is A: viral DNA polymerase. Herpesviruses require viral DNA polymerase for viral DNA replication. This enzyme is responsible for synthesizing new DNA strands using existing viral DNA as a template. Ribonucleotide reductase (B) is not directly involved in DNA replication but in the synthesis of deoxyribonucleotides. Neuraminidase (C) is an enzyme found in influenza viruses, not herpesviruses. Thymidine kinase (D) is involved in nucleotide metabolism but is not essential for viral DNA replication in herpesviruses.

Correct Answer: B

Rationale: The correct answer is B: Staphylococcus epidermidis. This bacterium is a normal skin flora commonly found on human skin. It is part of the skin microbiota and helps protect against pathogenic organisms. Streptococcus pyogenes (A), Pseudomonas aeruginosa (C), and Acinetobacter baumannii (D) are not normal skin flora. Streptococcus pyogenes is a pathogenic bacterium causing various infections, Pseudomonas aeruginosa is an opportunistic pathogen commonly found in water and soil, and Acinetobacter baumannii is associated with healthcare-associated infections.

Correct Answer: A

Rationale: The correct answer is A: Streptomycin. Streptomycin is an aminoglycoside antibiotic used to treat tuberculosis that can cause hearing loss as a side effect. It is known to damage the cochlear and vestibular organs in the inner ear. The other choices, B: Isoniazid, C: Rifampicin, and D: Ethionamide, are not associated with causing hearing deterioration as a side effect. Isoniazid can rarely cause peripheral neuropathy, Rifampicin can lead to hepatotoxicity, and Ethionamide may cause gastrointestinal disturbances, but none of these drugs are known to specifically cause hearing loss.

Correct Answer: C

Rationale: Rationale: Both Clostridium botulinum and Clostridium tetani produce neurotoxins. Botulinum toxin affects the nervous system by blocking neurotransmitter release, causing paralysis. Tetanus toxin affects the nervous system by interfering with neurotransmitter release, leading to muscle stiffness and spasms. Therefore, Choice C is correct. Choices A and B alone are incorrect because they only mention one of the bacteria that produces a neurotoxin. Choice D is incorrect because both bacteria mentioned in Choices A and B produce neurotoxins.

Correct Answer: A

Rationale: The correct answer is A: Shigella dysenteriae. This bacteria produces the Shiga toxin, which inhibits protein synthesis by interfering with the ribosomes. Shigella dysenteriae causes severe gastrointestinal illness. The other choices, B: Clostridium botulinum, produces a neurotoxin that blocks neurotransmitter release. C: Streptococcus pneumoniae causes pneumonia and other respiratory infections. D: Mycobacterium tuberculosis causes tuberculosis by infecting the lungs. Thus, based on the toxin production and mode of action, Shigella dysenteriae is the correct answer.

Correct Answer: A

Rationale: The correct answer is A: Genus Shigella. Shigella is known to be the causative agent of bacterial dysentery. It invades the intestinal lining, leading to symptoms like severe diarrhea and abdominal cramps. Salmonella (choice B) typically causes food poisoning, not dysentery. Klebsiella (choice C) is associated with pneumonia and urinary tract infections, not dysentery. "None of the above" (choice D) is incorrect as Shigella does belong to a specific genus responsible for bacterial dysentery.

Correct Answer: A

Rationale: The correct answer is A: McConkey agar. Selective media selectively inhibit the growth of certain organisms while allowing others to grow. McConkey agar contains crystal violet and bile salts that inhibit the growth of Gram-positive bacteria, making it selective for Gram-negative bacteria. Choices B and C are not selective media, as they do not specifically inhibit the growth of certain types of bacteria. Choice D is incorrect as McConkey agar is indeed an example of selective media.

Correct Answer: A

Rationale: The correct answer is A: Yersinia pestis. This bacterium causes the plague, which presents with fever and swollen lymph nodes. The safety pin appearance on Gram stain is characteristic of Yersinia pestis. B: Francisella tularensis causes tularemia, which presents differently and does not show safety pin appearance on Gram stain. C: Brucella abortus causes brucellosis, which does not typically present with the same symptoms as described in the question. D: Clostridium botulinum causes botulism, which does not show safety pin appearance on Gram stain and does not typically present with fever and swollen lymph nodes.