A child with a sore throat had a throat smear revealing Gram-positive cocci in pairs with a capsule. What is the most likely causative agent?

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Question 1 of 9

A child with a sore throat had a throat smear revealing Gram-positive cocci in pairs with a capsule. What is the most likely causative agent?

Correct Answer: A

Rationale: The correct answer is A: Streptococcus pneumoniae. The presence of Gram-positive cocci in pairs with a capsule is characteristic of Streptococcus pneumoniae. Step 1: Gram-positive cocci in pairs indicate a Streptococcus species. Step 2: The presence of a capsule suggests S. pneumoniae specifically. Summary: B: Staphylococcus aureus is a Gram-positive cocci but does not typically present in pairs with a capsule. C: Neisseria gonorrhoeae is a Gram-negative diplococcus, not a Gram-positive cocci. D: Klebsiella pneumoniae is a Gram-negative rod, not a cocci.

Question 2 of 9

A 45-year-old patient complains of body temperature rise up to 40oC, general weakness, headache, painfulness and spastic muscle contractions around the wound in the shin. He received this wound 5 days ago when working in his garden. He requested no medical care back then. What wound infection can be suspected?

Correct Answer: A

Rationale: The correct answer is A: Tetanus. The patient's symptoms of muscle contractions, fever, and wound history suggest tetanus, caused by Clostridium tetani bacteria commonly found in soil. The spastic muscle contractions around the wound are classic for tetanus due to the neurotoxin produced by the bacteria. General weakness and headache are also common symptoms. B: Anthrax is unlikely as it presents with different symptoms such as skin lesions, respiratory symptoms, and gastrointestinal symptoms. C: Erysipelas is characterized by red, swollen patches on the skin, not consistent with the symptoms described. D: Gram-positive is not a specific diagnosis but a broad category of bacteria. Tetanus is caused by Clostridium tetani, which is a Gram-positive bacterium, but this choice does not provide a specific infection diagnosis.

Question 3 of 9

What clinical specimen can be analysed in the diagnosis of sexually transmitted infections:

Correct Answer: D

Rationale: Certainly! The correct answer is D because all three options (A, B, and C) can be analyzed in the diagnosis of sexually transmitted infections. Genital ulcer secretions can reveal the presence of pathogens causing STIs, lymph node punctate can indicate systemic infection, and serum can be tested for specific STI antibodies. Therefore, all these clinical specimens are essential for a comprehensive diagnosis of sexually transmitted infections. The other choices (A, B, C) are incorrect as they individually represent important specimens used in the diagnosis process.

Question 4 of 9

Crude herbal drugs must be examined for yeast-like fungi. What agar can ensure development of these microorganisms so that associating microflora will grow very slowly or won't grow at all?

Correct Answer: A

Rationale: The correct answer is A: Sabouraud's peptone agar. This agar is specifically designed for the isolation of yeast and molds due to its low pH and high sugar content, creating an environment conducive for their growth. Yeast-like fungi thrive in this selective medium, while inhibiting the growth of bacteria and other contaminants. Summary: - Sabouraud's peptone agar is ideal for cultivating yeast-like fungi. - Endo agar is used for isolation of gram-negative bacteria, not fungi. - Milk-salt agar is used for lactobacilli and streptococci, not fungi. - Meat infusion agar is a general-purpose medium for cultivation of a wide range of microorganisms, not specifically for yeast-like fungi.

Question 5 of 9

A wound swab from a patient with severe tissue infection revealed Gram-positive rods in chains producing gas. The bacteria were spore-forming. What is the causative agent?

Correct Answer: A

Rationale: The correct answer is A: Clostridium perfringens. This bacterium is a Gram-positive rod that forms chains and produces gas due to its ability to ferment carbohydrates. It is also spore-forming, which helps it survive harsh conditions. Clostridium perfringens is commonly associated with severe tissue infections and gas gangrene. Choice B: Bacillus anthracis is also a spore-forming Gram-positive rod but is not typically associated with gas production or severe tissue infections. Choice C: Clostridium tetani is a spore-forming bacterium that causes tetanus, not severe tissue infections with gas production. Choice D: Pseudomonas aeruginosa is a Gram-negative bacterium and does not fit the description provided in the question.

Question 6 of 9

Exotoxins are:

Correct Answer: A

Rationale: Exotoxins are proteins produced inside the microbe cell and then released outside the cell to exert their toxic effects on neighboring cells or tissues. This is why choice A is correct. Choices B and C are incorrect because exotoxins are not meant to act within the same cell they are produced in. Choice D is incorrect because exotoxins are usually released without causing cell lysis; they are secreted by the microbe actively rather than being leaked out due to cell damage.

Question 7 of 9

For which family the following morphological features are typical: filamentous spiral nucleocapsid, linear unsegmented single strand (-) RNA and lipid bilayer membrane of cellular origin:

Correct Answer: B

Rationale: Step-by-step rationale: 1. Filamentous spiral nucleocapsid is characteristic of Filoviridae, such as Ebola virus. 2. Linear unsegmented single strand (-) RNA is a key feature of Filoviridae genomes. 3. The lipid bilayer membrane of cellular origin is found in Filoviridae viruses. 4. Therefore, the correct answer is B (Filoviridae) due to the alignment of all three morphological features. Summary: - A (Picornaviridae): Has an icosahedral capsid, not filamentous spiral. - C (Orthomyxoviridae): Has a segmented genome, not linear unsegmented. - D (Togaviridae): Has a spherical capsid, not filamentous spiral.

Question 8 of 9

Which of the following bacteria is known for producing a neurotoxin that causes botulism?

Correct Answer: A

Rationale: The correct answer is A: Clostridium botulinum. This bacterium produces the neurotoxin responsible for botulism. Clostridium perfringens is associated with gas gangrene, not botulism. Streptococcus pneumoniae causes pneumonia, and Neisseria gonorrhoeae causes gonorrhea, not botulism. Therefore, A is the correct choice.

Question 9 of 9

Which bacteria are capable of surviving in anoxic (oxygen-free) conditions?

Correct Answer: A

Rationale: The correct answer is A: Obligate anaerobes. These bacteria can only survive in the absence of oxygen as they lack the enzymes needed for aerobic respiration. They typically use fermentation or anaerobic respiration for energy production. Incorrect choices: B: Facultative anaerobes can switch between aerobic and anaerobic metabolism based on oxygen availability. C: Obligate aerobes require oxygen for survival and cannot survive in anoxic conditions. D: Microaerophiles require low levels of oxygen for growth and are not capable of surviving in completely anoxic conditions.

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