A 70-year-old man was admitted to the hospital after suffering extensive third-degree burns. Three days later he spiked a fever and there was pus on the dressing that had blue-green colour. Gram stain of the pus revealed gram-negative rods. The most possible cause of these findings is:

Correct Answer: A

Rationale: The correct answer is A: The pyocyanin released by Pseudomonas aeruginosa. Pyocyanin is a blue-green pigment produced by Pseudomonas aeruginosa, a common cause of nosocomial infections in burn patients. The presence of blue-green pus and gram-negative rods in the wound along with fever suggests an infection with Pseudomonas aeruginosa. The other choices are incorrect because E. Coli typically causes gastrointestinal infections and releases enterotoxins, Staphylococcus aureus releases hemolysins causing hemolysis of red blood cells, and Shigella dysenteriae releases shigatoxin causing bloody diarrhea, none of which match the clinical presentation described in the question.

Correct Answer: B

Rationale: The correct answer is B because transformation in bacteria refers to the uptake of naked DNA from the environment. During transformation, bacteria take up free DNA from their surroundings and incorporate it into their own genome. This mechanism allows bacteria to acquire new genetic material and traits. Explanation for other choices: A: Transfer of DNA via phages refers to transduction, not transformation. C: Transfer of DNA via direct contact is known as conjugation, not transformation. D: Spore formation is a method of bacterial survival but is not related to transformation.

Correct Answer: C

Rationale: The correct answer is C: All answers are correct. Pneumonia can be caused by various pathogens, including Legionella pneumophila and Mycoplasma pneumoniae. Choice C is correct because both A and B are known causative agents of pneumonia. Choice D is incorrect because we know that Legionella pneumophila and Mycoplasma pneumoniae can cause pneumonia, so the statement "None of the above" is not accurate in this context.

Correct Answer: A

Rationale: The correct answer is A: A vaccine strain. Virus Fixe is a specific strain of rabies virus used in the production of rabies vaccines. This strain has been attenuated to be safe for use in vaccines. Choice B, a cell culture, is incorrect as it does not specifically refer to Virus Fixe. Choice C, a wild type of rabies virus, is incorrect as Virus Fixe is not a wild type but a modified strain. Choice D, an infectious disease, is incorrect as Virus Fixe is not a disease but a strain of virus used in vaccines.

Correct Answer: B

Rationale: The correct answer is B because intensity, frequency, and thickness of the sample are key factors that determine the effects of different kinds of radiation. Intensity refers to the amount of energy carried by the radiation, frequency indicates the number of waves per unit time, and the thickness of the sample affects the penetration of radiation. These factors directly influence the interactions of radiation with matter, such as ionization and heating effects. A is incorrect because wavelength is not a major factor in determining the effects of radiation. C is incorrect because distance and nature of the sample are not as significant as intensity, frequency, and thickness. D is incorrect because chemical composition and shielding, while important in certain contexts, are not the primary factors affecting the effects of radiation.

Correct Answer: C

Rationale: Rationale: 1. The male reproductive system is considered sterile as it does not have a natural microbiota. 2. Any presence of microorganisms in this system could indicate an infection or disease. 3. Therefore, choice C, sterile with no normal flora, is the best characterization. Summary: A: Incorrect - The male reproductive system does not typically have gram-positive rods. B: Incorrect - Acid-fast rods are not part of the normal flora in the male reproductive system. D: Incorrect - The male reproductive system does not typically have gram-positive spore formers.

Correct Answer: A

Rationale: The correct answer is A: protein envelope around the nucleic acid. Viral capsid is the protein coat that surrounds and protects the viral nucleic acid (DNA or RNA). This structure helps in protecting the genetic material from degradation and facilitates the attachment of the virus to host cells. Option B is incorrect as it suggests the nucleic acid is coated with protein, which is not the case. Option C is incorrect as not all viruses have an outer membrane. Option D is incorrect as viruses typically do not have a lipoprotein membrane.

Correct Answer: D

Rationale: The correct answer is D, "All of the above," because all three bacteria (Salmonella enterica, Clostridium botulinum, Escherichia coli) are known to cause foodborne illnesses. Salmonella causes symptoms like diarrhea, fever, and abdominal cramps. Clostridium botulinum produces a toxin leading to botulism, a severe illness affecting the nervous system. Escherichia coli strains like E. coli O157:H7 can cause food poisoning with symptoms such as bloody diarrhea. Therefore, all the listed bacteria can cause foodborne illnesses due to their ability to contaminate food and infect individuals.

Correct Answer: A

Rationale: The correct answer is A: Sabouraud. Sabouraud agar is specifically designed to promote the growth of fungi due to its low pH and high glucose content. The cream-like colonies indicate fungal growth, as fungi often appear as fluffy or powdery colonies on Sabouraud agar. Lowenstein-Jensen is used for mycobacteria, Roux for diphtheria, and Loeffler for Corynebacterium diphtheriae. Therefore, Sabouraud is the most suitable medium for detecting fungal contamination based on the characteristics of the colonies formed.