A 42-year-old female has foamy purulent vaginal discharges. The smear stained by Romanovsky- Giemsa's method has been found to include flagellated bacteria. What is the most likely microorganism that has been found by the doctor?

Correct Answer: A

Rationale: The correct answer is A: Trichomonas vaginalis. Trichomonas vaginalis is a flagellated protozoan parasite commonly found in vaginal infections. The presence of flagellated bacteria in the vaginal discharge stained with Romanovsky-Giemsa method indicates a protozoan infection, ruling out bacterial or viral causes. Choices B, C, and D are not correct as Leishmania donovani causes leishmaniasis, Trypanosoma gambiense causes African sleeping sickness, and Trihomonas hominis is a non-pathogenic flagellate found in the human intestine, not typically associated with vaginal infections.

Correct Answer: A

Rationale: Rationale for Correct Answer A (Lamblia): 1. Pyriform protozoa with twin nuclei, four pairs of flagella, and a suctorial disc match the characteristics of Giardia lamblia. 2. The presence of two supporting filaments between the nuclei is a unique feature of Giardia lamblia. 3. Giardia lamblia is known to infect the duodenum and cause symptoms such as diarrhea and abdominal pain. Summary of Incorrect Choices: B: Toxoplasma - Toxoplasma gondii is not characterized by four pairs of flagella or supporting filaments in the nuclei. C: Leishmania - Leishmania species do not typically have the described morphology or location in the duodenum. D: Intestinal trichomonad - Intestinal trichomonads do not exhibit the specific features mentioned in the question, such as twin nuclei and supporting filaments.

Correct Answer: B

Rationale: The correct answer is B: Bergey's Manual of Systematic Bacteriology. This manual is a comprehensive and authoritative resource specifically dedicated to bacterial taxonomy, classification, and identification. It provides detailed descriptions and classification schemes for bacteria, aiding in accurate identification and understanding of bacterial species. Gray's Anatomy (choice A) focuses on human anatomy, the WHO Manual (choice C) focuses on global health guidelines, and Darwin's Origin of Species (choice D) discusses evolution and natural selection, none of which are specialized taxonomic resources for bacteria.

Correct Answer: C

Rationale: The correct answer is C: peroxisomes. Peroxisomes contain enzymes involved in breaking down fatty acids and detoxifying harmful substances. They do not contain digestive enzymes. Lysosomes contain digestive enzymes involved in breaking down waste materials. Chloroplasts are involved in photosynthesis and do not contain digestive enzymes. Mitochondria are involved in energy production and do not contain digestive enzymes. Therefore, the correct answer is peroxisomes.

Correct Answer: A

Rationale: The correct answer is A: HIV. HIV is an example of obligate parasitism because it requires a host cell to replicate and survive. It cannot live independently outside of a host organism. In contrast, choices B, C, and D are not obligate parasites. E. Coli and S. Epidermidis are examples of commensal bacteria that can live in the host without causing harm. Pseudomonas aeruginosa can be both an opportunistic pathogen and a free-living organism, not strictly dependent on a host for survival.

Correct Answer: A

Rationale: Step-by-step rationale for why A is correct: 1. Clostridium tetani is known to produce tetanus toxin. 2. The tetanus toxin causes muscle rigidity and spasms characteristic of tetanus. 3. Clostridium perfringens is associated with gas gangrene, not tetanus. 4. Neisseria gonorrhoeae causes gonorrhea, not tetanus. 5. Bacillus anthracis causes anthrax, not tetanus. In summary, Clostridium tetani is the correct answer as it is specifically associated with causing tetanus, while the other choices are associated with different diseases.

Correct Answer: A

Rationale: The most likely causative agent is Corynebacterium diphtheriae. Step 1: Presence of rod-shaped bacteria indicates a bacillus. Step 2: Polar granules suggest metachromatic granules seen in C. diphtheriae. Step 3: C. diphtheriae causes diphtheria, which presents with sore throat. Summary: B is incorrect as S. pneumoniae is a cocci, not a bacillus. C is incorrect as H. influenzae is a pleomorphic coccobacillus. D is incorrect as K. pneumoniae is a large, encapsulated bacillus.

Correct Answer: B

Rationale: Rationale: 1. Enteropathogenic E.coli (EPEC) adheres to the intestinal wall using bundle-forming pili. 2. EPEC produces toxins that disrupt cell structure and function, leading to diarrhea. 3. Shigella spp. invade intestinal cells, Salmonella spp. invade and multiply within cells, and Vibrio cholerae produces cholera toxin causing profuse watery diarrhea. Summary: A, C, and D choices are incorrect because they do not specifically adhere to the intestine wall and produce toxins like EPEC does.

Correct Answer: A

Rationale: The correct answer is A: Blood serum. In suspected cases of brucellosis, blood serum is typically analyzed in a common microbiological laboratory. This is because the bacteria causing brucellosis, Brucella species, can be detected through serological tests in the blood serum. The presence of specific antibodies against Brucella in the serum confirms the diagnosis. Spinal fluid (choice B) would be analyzed in cases of suspected central nervous system infections, which are not typical for brucellosis. Vomit mass (choice C) is not a common sample for microbiological analysis in cases of brucellosis. Urine (choice D) is not the preferred sample for detecting Brucella antibodies; blood serum is the primary choice for serological testing.