A 40 year old man noticed a reddening and an edema of skin in the area of his neck that later developed into a small abscess. The incised focus is dense, yellowish-green. The pus contains white granules. Histological examination revealed drusen of a fungus, plasmatic and xanthome cells, macrophages. What type of mycosis is the most probable?

Correct Answer: D

Rationale: The correct answer is D: Sporotrichosis. Sporotrichosis is caused by the fungus Sporothrix schenckii, which commonly presents as a subcutaneous infection. In the given case, the presence of a small abscess with yellowish-green pus containing white granules is indicative of sporotrichosis. The histological examination showing drusen of a fungus, plasmatic and xanthome cells, and macrophages further supports the diagnosis of sporotrichosis. Choice A: Actinomycosis is a bacterial infection caused by Actinomyces species, not a fungal infection. Choice B: Aspergillosis is caused by Aspergillus species and typically presents as a pulmonary infection, not a subcutaneous abscess as described in the case. Choice C: Candidosis is caused by Candida species and usually presents as mucocutaneous infections, not a subcutaneous abscess with the

Correct Answer: C

Rationale: The correct answer is C: Vibrio. Cholera is caused by Vibrio cholerae bacteria. These bacteria produce a toxin that causes severe diarrhea and dehydration. Escherichia (choice A) and Salmonella (choice B) are known for causing other types of gastrointestinal infections but not cholera. Acinetobacter (choice D) typically causes respiratory infections, not gastrointestinal illnesses like cholera. Therefore, the correct choice is Vibrio as it is specifically associated with cholera.

Correct Answer: B

Rationale: The correct answer is B: Gram's staining method. The rationale for this is as follows: Gentian violet and Lugol's solution are used as primary and mordant stains in the Gram's staining method. Spiritus 960 is used as a decolorizer, and water fuchsin is used as a counterstain. This staining method is commonly used in microbiology to differentiate bacteria into Gram-positive and Gram-negative based on their cell wall characteristics. A: Leffler's staining method is used for the identification of diphtheria bacilli. C: Ziehl-Neelsen's staining method is used for acid-fast bacteria like Mycobacterium tuberculosis. D: Neisser's staining method is used for the identification of Neisseria species. In summary, the use of specific reactives and stainers in the question indicates the application of the Gram's staining method for bacterial differentiation.

Correct Answer: D

Rationale: Binary fission is the correct answer for bacterial replication because it is the process by which bacteria divide into two identical daughter cells. During binary fission, the bacterial cell replicates its DNA, forms a septum dividing the cell into two, and eventually splits into two daughter cells. This process is specific to prokaryotic cells such as bacteria and is different from mitosis and meiosis, which are processes specific to eukaryotic cells. Cytokinesis is the final step of cell division in both eukaryotes and prokaryotes, but it is not the primary mechanism of bacterial replication.

Correct Answer: A

Rationale: The correct answer is A: Psittacosis. This disease is transmitted via aerosols containing dried bird droppings or respiratory secretions from infected birds. When these aerosols are inhaled, the bacteria causing psittacosis enter the respiratory system and infect the individual. - B: Shigellosis is transmitted through contaminated food or water, not via aerosols. - C: Leptospirosis is transmitted through contact with water, soil, or food contaminated with the urine of infected animals, not via aerosols. - D: Giardiasis is transmitted through ingesting contaminated food or water, not via aerosols. Therefore, Psittacosis is the only disease in the list that is transmitted via aerosols, making it the correct answer.

Correct Answer: A

Rationale: The correct answer is A: Entamoeba histolytica. This protozoan parasite causes amoebic dysentery and is characterized by cysts with four nuclei in fecal smears. It is commonly associated with chronic diarrhea. Balantidium coli (B) presents with large ciliated trophozoites, not cysts. Giardia lamblia (C) has trophozoites with flagella and causes giardiasis, not cysts with four nuclei. Trichomonas hominis (D) typically has a pear-shaped trophozoite and is not associated with cysts with four nuclei.

Correct Answer: D

Rationale: The most likely diagnosis is D: Lymphocutaneous tularemia. The key clues include exposure to a dead animal, vesicle with serosanginous exudate, painless edema progressing to lymph node involvement, and fever. Tularemia is caused by Francisella tularensis, commonly acquired through contact with infected animals. The characteristic ulcer at the site of entry, lymphadenopathy, and systemic symptoms point towards tularemia. Cutaneous anthrax (A) presents with a painless ulcer and black eschar. Bubonic plague (B) typically presents with buboes (swollen lymph nodes) and fever. Carbuncle (C) is a deep skin infection typically caused by Staphylococcus aureus.

Correct Answer: A

Rationale: The correct answer is A: Spores. In the Ozheshko staining method, spores appear blue with red terminal components. Spores are reproductive structures produced by some bacteria, fungi, and protozoa. Cilia are short, hair-like structures used for movement. Flagella are long, whip-like structures also used for movement. Capsules are outer protective layers surrounding some bacteria. In this case, the description of rod-like microorganisms with blue staining and red terminal components aligns with the characteristics of spores, making it the correct answer.

Correct Answer: D

Rationale: The correct answer is D because HIV can be transmitted from a mother to a child through multiple modes: during delivery through mixing of blood, during pregnancy through the placenta, and via breastmilk during lactation. Each mode presents a risk of transmission due to the presence of the virus in bodily fluids. Therefore, all of the above options are preferred modes of transmission for HIV from a mother to a child. Choices A, B, and C alone do not fully encompass all the possible routes of transmission, making them incorrect.