A 36-year-old man provisionally diagnosed with renal tuberculosis has undergone urinary sediment analysis. Microscopy revealed acid-fast bacteria, but Pryce method detected no cord factor. Name the most reliable method of investigation that can confirm or refute this provisional diagnosis:

Correct Answer: B

Rationale: The correct answer is B: Phage typing of the obtained culture. This method is used for typing Mycobacterium tuberculosis strains based on susceptibility to specific bacteriophages. If the culture shows susceptibility, it confirms the presence of M. tuberculosis. In this case, the absence of cord factor indicates that the bacteria are unlikely to be M. tuberculosis. Option A (Inoculation of laboratory animals) is unethical and not necessary when other methods are available. Option C (Allergy skin test) is not relevant for confirming a diagnosis of renal tuberculosis. Option D (Toxigenicity testing) is not specific for confirming or refuting a diagnosis of M. tuberculosis.

Correct Answer: A

Rationale: The correct answer is A: Anaerobic. Pulpitis is an inflammatory condition of the dental pulp, often caused by bacterial infection. Anaerobic bacteria are commonly associated with dental infections, making it likely to find them in the inoculated culture medium. Anaerobic bacteria thrive in the absence of oxygen, which is typical of the dental environment. Acid-resistant (B) and acidophilic (C) microorganisms are not specifically linked to pulpitis. Haemolytic (D) microorganisms typically lyse red blood cells, which is not a characteristic feature of bacteria causing pulpitis. Therefore, the most likely microorganisms to be found in the culture medium would be anaerobic bacteria.

Correct Answer: C

Rationale: The correct answer is C: Binding of mast cell-fixed IgE. The Bezredka method involves introducing small amounts of the antigen to which the individual is allergic, leading to the binding of mast cell-fixed IgE. This process desensitizes the immune system and prevents anaphylactic shock by reducing the release of histamine and other mediators from mast cells. Rationale: 1. A: Stimulation of antigen-specific IgG2 is incorrect because the Bezredka method primarily targets IgE, not IgG2. 2. B: Stimulation of the immunological antigen tolerance is incorrect as this method aims to block the allergic response rather than induce tolerance. 3. D: Blocking of mast cell mediators synthesis is incorrect because the Bezredka method does not directly inhibit the synthesis of mast cell mediators, but rather blocks their release by binding IgE.

Correct Answer: A

Rationale: The correct answer is A: Antitoxic passive immunity. Antibotulinic serum contains pre-formed antibodies against the botulinum toxin, providing immediate protection to the patient. This is passive immunity as the patient does not produce their antibodies but receives them externally. Choice B is incorrect as botulism is not an infection, so the patient does not develop infection immunity. Choice C is incorrect because active immunity involves the body producing its antibodies after exposure to an antigen, which is not the case here. Choice D is incorrect as antimicrobic immunity relates to protection against microbes, not toxins like in botulism.

Correct Answer: A

Rationale: The correct answer is A: Treponema. Treponema pallidum is a spirochete bacterium that appears as long, thin, and spiral-shaped microorganisms with 8-12 regular coils under the microscope. This characteristic morphology is specific to Treponema species, making it the correct choice. Borrelia, Leptospira, and Vibrios have different shapes and arrangements under the microscope, making them incorrect choices in this scenario.