A 3 month old infant has got a white deposition on the mucous membrane of his mouth, tongue and lips. The doctor suspected candidosis. What nutrient medium should be used for inoculation of the material under examination in order to confirm this diagnosis?

Correct Answer: A

Rationale: The correct answer is A: Sabouraud. Sabouraud agar is specifically designed for the cultivation of fungi and yeasts, making it ideal for identifying Candida species causing candidosis. It contains antibiotics to inhibit bacterial growth, promoting the growth of fungi. Endo, Loewenstein-Jensen, and Roux are not suitable for fungal cultures and are used for different purposes like bacterial isolation or mycobacterial cultures. Sabouraud agar is the best choice for confirming candidosis due to its fungal-selective properties.

Correct Answer: B

Rationale: The correct answer is B: Precipitins. When a granular sediment forms at the bottom of the test-tubes, it indicates the formation of antigen-antibody complexes that are insoluble and precipitate out of the solution. Precipitins are antibodies that specifically react with antigens to form insoluble complexes. Agglutinins cause clumping of cells, opsonins enhance phagocytosis, and bacteriolysins lyse bacteria. These processes do not result in the formation of a granular sediment, making them incorrect choices in this context.

Correct Answer: D

Rationale: The correct answer is D: All are correct. Culture of samples from the respiratory tract can identify specific pathogens, Immunofluorescence can detect viral antigens, and PCR can amplify and detect specific DNA/RNA sequences. Using all methods together provides a comprehensive approach for diagnosing respiratory tract infections. Culture alone may miss fastidious organisms, Immunofluorescence has limitations in sensitivity, and PCR requires specific primers. Therefore, combining these methods increases the likelihood of accurate diagnosis by covering a range of pathogens and improving sensitivity and specificity.

Correct Answer: A

Rationale: The correct answer is A: Trichomonas vaginalis. Trichomonas vaginalis is a protozoan parasite that causes trichomoniasis, a sexually transmitted infection characterized by inflammation of urogenital tracts. The description of big unicellular pyriform organisms with a sharp spike on the back end of their bodies, big nucleus, and undulating membrane matches the morphology of Trichomonas vaginalis. Trichomonas hominis and Trichomonas buccalis are different species that do not cause urogenital infections. Trypanosoma gambiense is a protozoan parasite causing African trypanosomiasis and does not match the described morphology or symptoms.

Correct Answer: A

Rationale: Ribosomes are the target for antibiotics as they interfere with protein synthesis in bacteria. Aminoglycosides, tetracyclines, macrolides, and lincosamides all target ribosomes by inhibiting protein synthesis at different stages. Beta-lactam antibiotics target cell wall synthesis, not ribosomes. Antifolate compounds, quinolones, and raspicin target DNA synthesis and replication, not ribosomes. Therefore, the correct answer is A as it includes antibiotics that specifically target ribosomes for inhibiting bacterial protein synthesis.

Correct Answer: B

Rationale: Step-by-step rationale for why choice B (Tuberculin is introduced) is correct: 1. Tuberculin test helps identify individuals with latent TB infection. 2. Negative skin-allergic samples suggest absence of active TB. 3. Introducing tuberculin can help confirm latent TB infection. 4. Antibiotics are not prescribed for negative skin-allergic samples. 5. Vaccines are not administered for TB diagnosis. Summary: - Choice A is incorrect as antibiotics are not prescribed for negative skin-allergic samples. - Choice C is incorrect as vaccines are not used for TB diagnosis. - Choice D is incorrect as introducing tuberculin is the appropriate step in this scenario.

Correct Answer: A

Rationale: The correct answer is A: destruction of the local blood supply. In osteomyelitis, the infection occurs in the bone, which has a relatively poor blood supply compared to soft tissues. This limited blood flow makes it challenging for antibiotics to reach the infection site effectively, hindering their ability to combat the infection. Destruction of the local blood supply restricts the delivery of antibiotics and immune cells to the infected bone, leading to poor treatment outcomes. Summary: - Option A is correct because the limited blood supply in bones hinders antibiotic delivery. - Option B is incorrect as denaturing by normal flora does not impact antibiotic reach to the infection site. - Option C is incorrect as inflammation and edema, while present, do not primarily hinder antibiotic delivery. - Option D is incorrect as excessive pus production does not directly impede antibiotic access to the infection site.

Correct Answer: B

Rationale: The correct answer is B: Alpha-hemolytic streptococci. Streptococcus pneumoniae are classified as alpha-hemolytic streptococci based on their ability to partially break down red blood cells, causing a greenish discoloration on blood agar plates. This distinguishes them from beta-hemolytic streptococci, which completely break down red blood cells. Streptococcus pneumoniae are not resistant to bile (choice A), as they are bile soluble. They are not stained by the Ziehl-Neelsen staining method (choice C), which is used for acid-fast bacteria such as Mycobacterium tuberculosis. They are sensitive to optochin (choice D), which is a key differentiating factor in identifying them from other alpha-hemolytic streptococci.

Correct Answer: D

Rationale: The correct answer is D: Monoclonal antibodies to anthrax causative agent. Monoclonal antibodies are highly specific for detecting anthrax spores, providing quick and accurate results. They bind only to the anthrax causative agent, allowing for precise identification. Choice A (Anti-anthrax fluorescent serum) is incorrect because it is not as specific and sensitive as monoclonal antibodies. Choice B (Standard anthrax antigen) is incorrect as it is used to stimulate antibody production in the body, not for direct detection of spores. Choice C (Anti-anthrax immunoglobulin) is incorrect as it is a general term for antibodies and may not be specific to anthrax spores. In summary, monoclonal antibodies are the most suitable option for quick and accurate detection of anthrax spores due to their high specificity and sensitivity.