Correct Answer: A

Rationale: The correct answer is A: Anaerobic infection. The sudden deterioration, bursting pain, edema, foul appearance of wound, and presence of gas are indicative of gas gangrene caused by anaerobic bacteria like Clostridium perfringens. These bacteria thrive in low oxygen environments, such as deep wound tissues. The symptoms align with the classic presentation of gas gangrene, necessitating immediate surgical intervention. Choice B: Aerobic gram-negative, is incorrect because anaerobic bacteria, not aerobic gram-negative bacteria, are responsible for gas gangrene. Choice C: Putrid, is incorrect as it is a general term referring to foul-smelling decay and does not specify the type of infection. Choice D: Aerobic gram-positive, is incorrect as gas gangrene is typically caused by anaerobic bacteria, not aerobic gram-positive bacteria.

Correct Answer: A

Rationale: The correct answer is A because in the lytic cycle, the bacteriophage destroys the host cell upon release, whereas in the lysogenic cycle, the phage integrates its DNA into the host genome without killing the host immediately. This integration may lead to the destruction of the host cell at a later stage. Choice B is incorrect as lytic phages do destroy the host cell. Choice C is incorrect as the two cycles have distinct mechanisms. Choice D is incorrect as there are clear differences between the lytic and lysogenic cycles.

Correct Answer: A

Rationale: The correct answer is A: CMV (Cytomegalovirus). CMV is the most common congenital infection worldwide due to its high prevalence and ability to be transmitted from mother to fetus during pregnancy. It can lead to serious complications such as hearing loss, developmental delays, and vision problems in newborns. Listeria monocytogenes (B) is a foodborne pathogen, not typically associated with congenital infections. Rubella Virus (C) can cause congenital rubella syndrome but is less common globally compared to CMV. Syphilis (D) can also be transmitted from mother to fetus, but its prevalence and impact on congenital infections are lower compared to CMV.

Correct Answer: A

Rationale: The correct answer is A: Ziehl-Neelsen staining method was applied. 1. Ziehl's solution is used as a primary stain in Ziehl-Neelsen staining for acid-fast organisms like Mycobacterium tuberculosis. 2. Sulfuric acid is used as a decolorizer to remove excess stain. 3. Methylene blue is not part of the Ziehl-Neelsen staining method. 4. Burri's, Gram's, and Peshkov's staining methods are not used for acid-fast staining.

Correct Answer: D

Rationale: The correct answer is D because all three bacteria mentioned (Clostridium botulinum, Vibrio cholerae, and Escherichia coli) are capable of producing exotoxins that cause severe diarrhea. Clostridium botulinum produces botulinum toxin leading to botulism, Vibrio cholerae produces cholera toxin causing cholera, and some strains of Escherichia coli produce enterotoxins causing severe diarrhea. Choices A, B, and C alone are incorrect because each of them can produce exotoxins that cause severe diarrhea.