During the incubation period S. Typhi multiplies in the

Correct Answer: D

Rationale: The correct answer is D because during the incubation period of Salmonella Typhi infection, the bacteria multiply in the macrophages of the Peyer's patches and solitary follicles. This is supported by the fact that S. Typhi is an intracellular pathogen that targets the lymphoid tissues of the intestines. Macrophages in these areas serve as the primary host cells for the bacteria, allowing for their replication and evasion of the host immune response. Choices A, B, and C are incorrect because S. Typhi does not primarily multiply in the small intestine enterocytes, the lumen of the small intestine, or hepatocytes during the initial stages of infection.

Correct Answer: B

Rationale: The correct answer is B: cytopathic effect. When viruses infect cell cultures, they induce degenerative changes known as cytopathic effects, which include cell shrinkage, rounding, detachment, and cell death. This term specifically refers to the damage caused by viral replication within the cells. A: Cytolytic effect refers to the destruction of cells by viruses, which is a more specific type of cytopathic effect involving cell lysis. C: Cytotoxic effect typically refers to the toxic effects of substances on cells, not specifically related to viral propagation. D: The statement that all three answers are correct is incorrect as only option B accurately describes the degenerative changes resulting from viral propagation in infected cell cultures.

Correct Answer: B

Rationale: The correct answer is B: Family Reoviridae. Reassortment of gene segments occurs in segmented viruses like Reoviruses. In Reoviridae, each segment encodes a specific protein, allowing for reassortment to generate new strains. Togaviridae (A) is a non-segmented virus, not capable of reassortment. Picornaviridae (C) and Paramyxoviruses (D) are also non-segmented viruses, limiting their ability for reassortment. Therefore, the correct choice is B due to the segmented nature of Reoviruses allowing for genetic reassortment.

Correct Answer: B

Rationale: The correct answer is B: Hepatitis B virus. This is because Hepatitis B virus is a DNA virus, not an RNA virus. The other choices, A (Hepatitis G virus), C (Hepatitis A virus), and D (Hepatitis E virus) are all RNA viruses. Hepatitis B virus is unique as it contains partially double-stranded DNA. DNA viruses replicate through a DNA intermediate, unlike RNA viruses which directly use RNA. Therefore, Hepatitis B virus stands out as the only DNA virus among the given options.

Correct Answer: A

Rationale: The correct answer is A: Lowestein-Jensen. This is because Lowestein-Jensen medium is specifically designed to cultivate Mycobacterium tuberculosis, providing necessary nutrients and conditions for its growth. Loefller's medium is not optimal for cultivating M. tuberculosis. Choice C (All of the above) is incorrect as not all the options listed are suitable for cultivating M. tuberculosis. Choice D (None of the above) is also incorrect as Lowestein-Jensen is indeed the recommended growth medium for this bacterium.